Transport, manipulation, selection and assay of single living cells

单个活细胞的运输、操作、选择和测定

基本信息

  • 批准号:
    138281-2011
  • 负责人:
  • 金额:
    $ 1.68万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2011
  • 资助国家:
    加拿大
  • 起止时间:
    2011-01-01 至 2012-12-31
  • 项目状态:
    已结题

项目摘要

Scientific progress in medicine has allowed health care professionals to scale their observations from systemic symptoms down to characterizing specific cell groups. This has resulted in more accurate, patient and disease-specific treatment plans that target precise pathologic processes. Thus, single cell assessment becomes the natural next step. The main objective of the proposed program is to advance the knowledge in development of microsystems that enable single cell assay. The short term objectives of the proposed program are directed towards development of models of physical phenomena that enable users to transport and accurately position targeted living cells separated from blood cells and development of microfluidics capable to accurately perform such tasks. Further, any enabling detection method that would help characterize the individual living cell from the biological, mechanical or electrical point of view will be considered. In long term, the principles used to separate, manipulate and position single cells will adjust to yield higher sensitivity and accuracy such as to produce less false negatives or positives. Meanwhile, the principles of cell separation, manipulation and positioning will enable development of microfluidics that could be used as separators, test devices, and incubators. The latter will enable real time sub-culture and evaluation of personalized treatment for certain diseases. Thus, the characterization of single cells after single cell trial treatment could yield valuable insight on the development of strategies in the development of personalized treatment. The short term research program focuses on engineering application to microfluidic and MEMS while the biology aspect is of concern but not a subject of investigation. The biology of single cells will become a subject of investigation in parallel with a microsystem development targeting a specific assay during the long term research program. The proposed research topic is interesting as it provides more insight into life sciences, and it is challenging for the engineering side as many of the issues related to this subject on modeling of the electric field and the interaction with media and living cells, as well as micro-fabrication techniques, are presently unknown.
医学的科学进步使医疗保健专业人员能够将他们的观察范围从全身症状扩展到特定细胞群的特征。这导致了针对精确病理过程的更准确、针对患者和疾病的治疗计划。因此,单细胞评估自然成为下一步。拟议计划的主要目标是增进单细胞检测微系统开发知识。该计划的短期目标是开发物理现象模型,使用户能够运输和准确定位与血细胞分离的目标活细胞,并开​​发能够准确执行此类任务的微流体。此外,将考虑任何有助于从生物、机械或电学角度表征单个活细胞的检测方法。从长远来看,用于分离、操作和定位单细胞的原理将进行调整,以产生更高的灵敏度和准确性,例如产生更少的假阴性或阳性。同时,细胞分离、操作和定位的原理将有助于开发可用作分离器、测试设备和培养箱的微流体。后者将能够实现实时亚培养和对某些疾病的个性化治疗的评估。因此,单细胞试验治疗后单细胞的表征可以为个性化治疗开发策略的制定提供有价值的见解。短期研究计划侧重于微流体和 MEMS 的工程应用,而生物学方面值得关注,但不是研究主题。在长期研究计划中,单细胞生物学将成为与针对特定测定的微系统开发并行的研究主题。拟议的研究主题很有趣,因为它提供了对生命科学的更多见解,并且对工程方面来说具有挑战性,因为与该主题有关的许多有关电场建模、与介质和活细胞相互作用以及微制造技术的问题目前尚不清楚。

项目成果

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专利数量(0)

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Stiharu, Ion其他文献

Vibration and noise reduction of pipelines using shape memory alloy
Bioenzymatic detection of troponin C using micro-opto-electro-mechanical systems
  • DOI:
    10.1117/1.2186326
  • 发表时间:
    2006-03-01
  • 期刊:
  • 影响因子:
    3.5
  • 作者:
    Amritsar, Jeetender;Stiharu, Ion;Packirisamy, Muthukumaran
  • 通讯作者:
    Packirisamy, Muthukumaran
Performance enhancement of cantilever piezoelectric energy harvesters by sizing analysis
Two-dimensional model of imbibition into paper-based networks using Richards' equation
  • DOI:
    10.1007/s10404-017-1937-0
  • 发表时间:
    2017-05-01
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    Perez-Cruz, Angel;Stiharu, Ion;Dominguez-Gonzalez, Aurelio
  • 通讯作者:
    Dominguez-Gonzalez, Aurelio
Synthesis and characterization of polyureasilazane derived SiCN ceramics
  • DOI:
    10.1063/1.2202291
  • 发表时间:
    2006-06-01
  • 期刊:
  • 影响因子:
    3.2
  • 作者:
    Andronenko, Sergey I.;Stiharu, Ion;Misra, Sushil K.
  • 通讯作者:
    Misra, Sushil K.

Stiharu, Ion的其他文献

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{{ truncateString('Stiharu, Ion', 18)}}的其他基金

Modeling, design and applications of MEMS
MEMS 建模、设计与应用
  • 批准号:
    RGPIN-2021-04029
  • 财政年份:
    2022
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
Modeling, design and applications of MEMS
MEMS 建模、设计与应用
  • 批准号:
    RGPIN-2021-04029
  • 财政年份:
    2021
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
Modeling, design, analysis and tests of MEMS
MEMS 建模、设计、分析和测试
  • 批准号:
    RGPIN-2016-06699
  • 财政年份:
    2020
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
Modeling, design, analysis and tests of MEMS
MEMS 建模、设计、分析和测试
  • 批准号:
    RGPIN-2016-06699
  • 财政年份:
    2019
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
Modeling, design, analysis and tests of MEMS
MEMS 建模、设计、分析和测试
  • 批准号:
    RGPIN-2016-06699
  • 财政年份:
    2018
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
Modeling, design, analysis and tests of MEMS
MEMS 建模、设计、分析和测试
  • 批准号:
    RGPIN-2016-06699
  • 财政年份:
    2017
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
Modeling, design, analysis and tests of MEMS
MEMS 建模、设计、分析和测试
  • 批准号:
    RGPIN-2016-06699
  • 财政年份:
    2016
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
L'Analyse de l'interaction entre les roues de sécurité et les lames d'aiguille
安全路线和戴吉耶拉姆斯之间的互动分析
  • 批准号:
    491143-2015
  • 财政年份:
    2015
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Engage Grants Program
MEMS for diagnostic and health monitoring of complex systems
用于复杂系统诊断和健康监测的 MEMS
  • 批准号:
    RGPIN-2014-06532
  • 财政年份:
    2014
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual
MEMS applications for single cell manipulation and assessment
用于单细胞操作和评估的 MEMS 应用
  • 批准号:
    138281-2012
  • 财政年份:
    2012
  • 资助金额:
    $ 1.68万
  • 项目类别:
    Discovery Grants Program - Individual

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