Functional charcterization of plant secondary metabolism/metabolites

植物次生代谢/代谢物的功能表征

• 批准号: 157930-2007
• 负责人: Bernards, Mark
• 金额: $ 2.91万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=476147
• 关键词: Functional; charcterization; plant; secondary; metabolism

Plant secondary metabolites are compounds that mediate interactions between plants and their environment. My research program explores (1) pathways involved in secondary metabolism and (2) the ecological roles of secondary metabolites. For example the study of suberin formation allows exploration of the regulation of metabolic pathways and the partitioning of carbon between different potential metabolic endpoints - critical knowledge required to make informed decisions about modifying plant metabolism for improved nutrition or enhanced disease resistance. Suberin is a tissue-specific cell wall modification deposited in a developmental manner as well as in response to wounding and plays a critical role in water retention and protection against pathogens. Using chemical, biochemical and metabolomic approaches my research group has identified key enzymatic steps unique to the biosynthesis of suberin. This proposal describes my intent to isolate and characterize the genes encoding these steps. While molecular biology tools are new to my research program, their use represents a logical progression in our approach to study suberin biosynthesis.

植物次生代谢物是介导植物与环境相互作用的化合物。我的研究项目探索（1）参与次生代谢的途径和（2）次生代谢物的生态作用。例如，木栓质形成的研究允许探索代谢途径的调节和碳在不同潜在代谢终点之间的分配-这是做出关于改变植物代谢以改善营养或增强抗病性的明智决定所需的关键知识。Suberin是一种组织特异性细胞壁修饰，以发育方式以及响应创伤沉积，并在保水和保护免受病原体侵害方面发挥关键作用。利用化学、生物化学和代谢组学方法，我的研究小组已经确定了木栓素生物合成所特有的关键酶促步骤。这个提议描述了我分离和描述编码这些步骤的基因的意图。虽然分子生物学工具对我的研究计划来说是新的，但它们的使用代表了我们研究木栓质生物合成方法的逻辑进展。