LC-MS for the Analysis of Small Molecules

我们正在寻求资金，以获得用于小分子分析的LC-MS系统的质谱仪组件。具体而言，我们正在申请资金购买Agilent Single Quad ES检测器。我们将通过配套1260 Infinity UHPLC系统的替代来源获得资金。内部LC-MS设施的可用性将支持七个申请人的研究计划。其中四个（Bernards，Huner和Grbics）将使用该系统进行植物激素分析。这些分子以非常低的丰度存在，但对整体代谢有显著影响（特别是在应对压力时）。LC-MS是植物激素分析中最灵敏和最合适的工具。其他申请人将使用该系统来分析植物对金属胁迫（Macfie）、细菌类霍帕诺（Valvano）和铁载体（海因里希斯）的响应中涉及的根分泌物。收集的数据将解决植物和细菌胁迫生物学的基本问题。

We are seeking funds to acquire the mass spectrometer component of an LC-MS system to be used for small molecule analysis. Specifically, we are asking for funds to purchase an Agilent Single Quad ES detector. We will acquire funds through an alternative source for the companion 1260 Infinity UHPLC system. The availability of in-house LC-MS facilities will support the research programs of seven applicants. Four of these (Bernards, Huner and the Grbics) will use the system for plant hormone analysis. These molecules are present in very low abundance, yet have significant impact on overall metabolism (especially in response to stress). LC-MS is the most sensitive and appropriate tool for plant hormone anlysis. Other applicants will use the system for the analysis of root exudates involved in plant response to metal stress (Macfie), bacterial hopanoids (Valvano) and siderophores (Heinrichs). The data collected will address fundamental questions of plant and bacterial stress biology.