Time of Flight Mass Spectrometer for GC-based analysis of small molecules

用于基于 GC 的小分子分析的飞行时间质谱仪

• 批准号: RTI-2020-00251
• 负责人: Bernards, Mark
• 金额: $ 10.93万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693834
• 关键词: Time; Flight; Mass; Spectrometer; GC

Small molecule analysis is an integral component of assessing biological mechanisms and can be either targeted or non-targeted. For targeted analysis, absolute or relative quantitation of specific molecules is achieved by optimizing chromatographic and detection conditions to the target molecules. In contrast, non-targeted analyses balance suboptimal chromatographic separation with high resolution mass analysis leading to informative mass-based deconvolution. Time of flight (ToF) mass spectrometers offer high resolution mass accuracy (approx. 1 ppm) allowing deconvolution of complex sample mixtures that are poorly resolved by chromatography. They are suitable for routine GC-MS analysis of a wide range of molecule classes, since they can generate high resolution accurate mass and EI mass spectra that can be compared with published data. GC-TOF-MS represents the industry standard for non-targeted (metabolomic) analysis of primary metabolites. In all the research programs highlighted in this proposal, the analysis of small molecules through either targeted or non-targeted approaches forms an integral part of the solution to larger biological questions. For example, the GC-ToF-MS system will be used to (1) discover biomarkers of altered primary metabolism in neurodegeneration using a mouse retina model, (2) identify potential biopesticides from tomato for the control of spider mite infestation, (3) track the flow of carbon through different trophic levels in a soil model of biodiversity, understand plant defenses against heavy metal soil contaminants, (4) understand carbohydrate dynamics in trees grown under future climate conditions and (5) determine the carbon flux in wound-induced potato tubers. These basic and applied projects reflect a broad range of topics, all with the common feature of requiring small molecule analysis to help establish underlying mechanisms, and thereby deeper understanding of the systems.

小分子分析是评估生物机制的一个组成部分，可以是靶向的也可以是非靶向的。对于靶向分析，通过优化靶分子的色谱和检测条件来实现特定分子的绝对或相对定量。相比之下，非靶向分析平衡了次优色谱分离与高分辨率质量分析，导致信息丰富的基于质量的反褶积。飞行时间（ToF）质谱仪提供高分辨率质量精度(约为。1 ppm)，允许反褶积复杂的样品混合物，通过色谱很难解决。它们适用于广泛分子类别的常规GC-MS分析，因为它们可以生成高分辨率准确的质量和EI质谱，可以与已发表的数据进行比较。GC-TOF-MS代表了初级代谢物的非靶向（代谢组学）分析的行业标准。在本提案中强调的所有研究项目中，通过靶向或非靶向方法对小分子进行分析是解决更大生物学问题的一个组成部分。例如，GC-ToF-MS系统将用于(1)利用小鼠视网膜模型发现神经退行性变中初级代谢改变的生物标志物，(2)从番茄中识别控制蜘蛛螨侵害的潜在生物农药，(3)在生物多样性土壤模型中跟踪碳在不同营养水平的流动，了解植物对重金属土壤污染物的防御。(4)了解在未来气候条件下生长的树木的碳水化合物动态；(5)确定伤致马铃薯块茎中的碳通量。这些基础和应用项目反映了广泛的主题，所有这些都有一个共同的特点，即需要小分子分析来帮助建立潜在的机制，从而更深入地了解系统。