Development biosensor for the detection of progesterone hormone as emerging contaminant in water

开发用于检测水中新兴污染物黄体酮激素的生物传感器

• 批准号: 412021-2010
• 负责人: Zourob, Mohammed
• 金额: $ 1.82万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=490762
• 关键词: Development; biosensor; detection; progesterone; hormone

The proposed project aims to develop a fluorescent optical biosensor for the detection of hormones in water. The biosensors developed in this project will be simple to use, portable and low-cost with superior sensitivity and specificity. The project involves the development of highly DNA-based capturing molecules targeting the hormone; followed by conjugation with fluorescent labelling moieties (fluorescent molecule and quencher) at both termini of the DNA. Characteristic fluorescent signal will be emitted upon the specific capturing of the targeted hormones by the labelled DNA. The signal is proportional to the concentration of the hormone in the water sample, thereby providing a quantitative detection. The technology generated in this project will be transferred to our industrial partner Lab-Bell and will be further developed into promising diagnostic tools for use in environmental pollution monitoring and water industry.

该项目旨在开发一种荧光光学生物传感器，用于检测水中的激素。本计画所开发之生物感测器将具有使用简单、携带方便、成本低廉、灵敏度及专一性均优于上级者。该项目涉及开发靶向激素的高度基于DNA的捕获分子;然后在DNA的两端与荧光标记部分（荧光分子和猝灭剂）缀合。当标记的DNA特异性捕获靶向激素时，将发射特征性荧光信号。该信号与水样中激素的浓度成比例，从而提供定量检测。该项目所产生的技术将转让给我们的工业合作伙伴Lab-Bell，并将进一步开发成用于环境污染监测和水工业的有前途的诊断工具。