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Development of endolysin-based biosensor for the detection of Lasteria bacteria contamination

开发基于细胞内溶素的生物传感器来检测拉斯特氏菌污染

基本信息

批准号：
412058-2010
负责人：
Zourob, Mohammed
金额：
$ 1.82万
依托单位：
Institut national de la recherche scientifique
依托单位国家：
加拿大
项目类别：
Engage Grants Program
财政年份：
2011
资助国家：
加拿大
起止时间：
2011-01-01 至 2012-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=490781
关键词：
Development endolysin based biosensor detection

项目摘要

The development of a sensitive, inexpensive biosensor to detect and quantify bacteria on-site in food and drinking water samples is an important scientific challenge. Current methods for bacteria detection are slow, inefficient and inadequate for taking timely action in case of outbreaks, which can result in tremendous economic burden and pose a serious risk to human health. There is a great demand for rapid and accurate detection/monitoring of bacteria in the food industry. We are focusing on the development of a portable, real-time and low-cost detection system targeting Listeria. This novel system utilizes a bacteriophage-based product to accomplish sensitive detection/capturing of Listeria. We will employ bacterial cell wall-binding proteins (CBD) derived from Listeria-specific phages as the recognition receptor in our detection system. The high-affinity/specificity binding of Listeria to the CBD will be detected by an impedance-based sensing device. This unique combination will result in a novel tool for the rapid detection of Listeria in food samples. This technology is not limited to bacteria detection in food industry, but also has wide applications in water resources, homeland security, environment pollution and point-of-care monitoring.

开发一种灵敏、廉价的生物传感器来检测和定量食品和饮用水样品中的细菌是一项重要的科学挑战。目前的细菌检测方法速度慢、效率低，不足以在爆发时及时采取行动，这可能导致巨大的经济负担，并对人类健康构成严重威胁。在食品工业中，对细菌的快速和准确检测/监测有很大的需求。我们正致力于开发一种便携式、实时、低成本的李斯特菌检测系统。该新系统利用基于噬菌体的产品来实现李斯特菌的灵敏检测/捕获。我们将采用细菌细胞壁结合蛋白（CBD）来源于李斯特菌特异性大肠杆菌作为识别受体在我们的检测系统。李斯特菌与CBD的高亲和力/特异性结合将通过基于阻抗的传感装置检测。这种独特的组合将产生一种快速检测食品样品中李斯特菌的新工具。该技术不仅限于食品工业中的细菌检测，而且在水资源，国土安全，环境污染和床旁监测方面也有广泛的应用。