Development of bar-coded, highly sensitive, label-free highthroughput screening platform for endocrine disrubting compounds

开发条形码、高灵敏度、无标记高通量内分泌干扰化合物筛选平台

• 批准号: 430261-2012
• 负责人: Zourob, Mohammed
• 金额: $ 14.1万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=516462
• 关键词: Development; bar; coded; highly; sensitive

Endocrine Disrupting Compounds (EDCs) are chemicals that may interfere with the body's endocrine system and produce adverse developmental, reproductive, neurological, and immune effects in both humans and wildlife. They can mimic naturally occurring hormones like estrogens and androgens, binding to receptors within cells, blocking endogenous hormones and causing interference with the production or control of natural hormones and their receptors. Health authorities are becoming more concerned about the potential public health impact of the EDCs originated from household and industrial practices. Although the concentrations of these contaminants are usually low in our water systems, there may induce adverse wildlife and human health effects (e.g. cancer risk and adverse reproductive development). Consequently, highly sensitive detection methods for these compounds are in demand. We aim to develop a novel detection technology that is suitable for routine monitoring of EDCs in water. This new monitoring system will be superior to current methodologies in terms of sensitivity, detection limit, throughput and sample processing. Our detection platform will consists of an array of bar-coded microsphere light resonators ( optical sensors) integrated with specific molecularly imprinted polymer (MIP) elements for multi-analyte high-throughput detection. Until now, the performance of EDC sensors is generally limited by their sensitivity and the use of biological recognition elements, such as antibodies or biochemical reactions. These biosensors share a common shortcoming of expensive reagents, which limits their use in routine water monitoring. In contrast, our novel detection platform has a high sensitivity and employs a class of synthetic recognition elements that we believe will be less expensive and hence cost-competitive against current detection methodologies.

内分泌干扰化合物（EDCs）是一种可能干扰人体内分泌系统并对人类和野生动物的发育、生殖、神经和免疫产生不良影响的化学物质。它们可以模仿天然存在的激素，如雌激素和雄激素，与细胞内的受体结合，阻断内源性激素并干扰天然激素及其受体的产生或控制。卫生当局越来越关注源自家庭和工业实践的内分泌干扰物对公共卫生的潜在影响。虽然这些污染物在我们的水系统中的浓度通常很低，但可能会对野生动物和人类健康产生不利影响（例如癌症风险和不利的生殖发育）。因此，需要这些化合物的高灵敏度检测方法。 我们的目标是开发一种新的检测技术，适用于水中内分泌干扰物的常规监测。这一新的监测系统在灵敏度、检出限、通量和样品处理方面将上级现有的方法。我们的检测平台将包括一个阵列的条形码微球光谐振器（光学传感器）集成特定的分子印迹聚合物（MIP）的多分析物的高通量检测元件。到目前为止，EDC传感器的性能通常受到其灵敏度和生物识别元件（如抗体或生化反应）的使用的限制。这些生物传感器都有一个共同的缺点，即昂贵的试剂，这限制了它们在日常水监测中的使用。相比之下，我们的新型检测平台具有高灵敏度，并采用了一类合成识别元素，我们认为这些元素将更便宜，因此与当前的检测方法相比具有成本竞争力。