"ISOLATION, CHARACTERIZATION AND USE OF SELF-ASSEMBLING MILK-DERIVED PEPTIDES IN THE SEPARATION OF PROTEIN HYDROLYSATES"

“自组装乳源肽在蛋白质水解物分离中的分离、表征和用途”

• 批准号: 36714-2012
• 负责人: Pouliot, Yves
• 金额: $ 2.26万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=506611
• 关键词: ISOLATION; CHARACTERIZATION; USE; SELF; ASSEMBLING

Milk and whey protein enzymatic hydrolysates have been widely used as a source of bioactive peptides (e.g. antihypertensive, immunomodulatory, etc.). However, developing potent extracts requires that the bioactive molecules are concentrated or even separated from these hydrolysates Our recent work has focused at a peptide fragment from beta-lactoglobulin (b-LG), namely b-LG f1-8, that has been isolated from aggregated material upon concentration of a tryptic hydrolysate of b-LG by reverse osmosis. This peptide sequence was found to have self-assembling properties that leads to the formation of nanofibers and hydrogels at specific concentration, pH and temperature values. In preliminary experiments, we have observed that contacting protein hydrolysates with nanofibers of peptide b-LG f1-8 could modify their peptide composition, suggesting that these nanofibers can adsorb hydrophobic peptides on their surface. The proposed work is based on the research hypothesis that it is possible to isolate milk-derived peptides from complex mixtures by inducing self-assembly. The peptide mixture pre-treated under these conditions will exhibit improved physicochemical stability and the self-assembling peptides can in turn be used in peptide separation. The long-term objective of our research is to develop innovative approaches for the isolation of peptides at large scale. More specifically, our work will focus at developing fundamental knowledge on structure-function characteristics of milk peptides in relation with their ability to form nanostructures that can be used as processing tools in peptide separation. The research program will be initiated using peptide b-LG f1-8 but it is expected that the work will lead to the discovery of other self-assembling peptides from whey proteins. This original approach is likely to open new possibilities for the separation of minor compounds such as bioactive peptides from milk. The research program will provide unique opportunities to train highly qualified personnel (HQP) who will possess a strong background in milk proteins and their processing into hydrolysates and bioactive peptides. This research has a very high potential to generate innovations that will be useful for the Canadian dairy industry.

牛奶和乳清蛋白酶解物已被广泛用作生物活性肽的来源（例如抗高血压，免疫调节等）。然而，开发有效的提取物需要将生物活性分子浓缩或甚至从这些水解物中分离出来。我们最近的工作集中在β -乳球蛋白（b-LG）的肽片段，即b-LG f1-8，该片段已通过反渗透从b-LG的色氨酸水解物浓缩后的聚集物中分离出来。该肽序列具有自组装特性，可在特定浓度、pH值和温度下形成纳米纤维和水凝胶。在初步实验中，我们观察到蛋白质水解物与肽b-LG f1-8纳米纤维接触可以改变其肽的组成，这表明这些纳米纤维可以在其表面吸附疏水肽。提出的工作是基于研究假设，有可能从复杂的混合物中分离牛奶衍生肽诱导自组装。在这些条件下预处理的多肽混合物将表现出更好的物理化学稳定性，并且自组装肽可以反过来用于多肽分离。我们研究的长期目标是开发大规模分离多肽的创新方法。更具体地说，我们的工作将集中在发展牛奶肽的结构-功能特征的基础知识，这些特征与它们形成纳米结构的能力有关，纳米结构可以用作肽分离的处理工具。该研究计划将从肽b-LG f1-8开始，但预计这项工作将导致从乳清蛋白中发现其他自组装肽。这种原始的方法很可能为从牛奶中分离少量化合物（如生物活性肽）开辟新的可能性。该研究项目将为培养在牛奶蛋白及其水解产物和生物活性肽加工方面具有强大背景的高素质人才（HQP）提供独特的机会。这项研究具有非常高的潜力，可以产生对加拿大乳制品行业有用的创新。