"ISOLATION, CHARACTERIZATION AND USE OF SELF-ASSEMBLING MILK-DERIVED PEPTIDES IN THE SEPARATION OF PROTEIN HYDROLYSATES"

“自组装乳源肽在蛋白质水解物分离中的分离、表征和用途”

• 批准号: 36714-2012
• 负责人: Pouliot, Yves
• 金额: $ 2.26万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=593034
• 关键词: ISOLATION; CHARACTERIZATION; USE; SELF; ASSEMBLING

Milk and whey protein enzymatic hydrolysates have been widely used as a source of bioactive peptides (e.g. antihypertensive, immunomodulatory, etc.). However, developing potent extracts requires that the bioactive molecules are concentrated or even separated from these hydrolysates Our recent work has focused at a peptide fragment from beta-lactoglobulin (b-LG), namely b-LG f1-8, that has been isolated from aggregated material upon concentration of a tryptic hydrolysate of b-LG by reverse osmosis. This peptide sequence was found to have self-assembling properties that leads to the formation of nanofibers and hydrogels at specific concentration, pH and temperature values. In preliminary experiments, we have observed that contacting protein hydrolysates with nanofibers of peptide b-LG f1-8 could modify their peptide composition, suggesting that these nanofibers can adsorb hydrophobic peptides on their surface. The proposed work is based on the research hypothesis that it is possible to isolate milk-derived peptides from complex mixtures by inducing self-assembly. The peptide mixture pre-treated under these conditions will exhibit improved physicochemical stability and the self-assembling peptides can in turn be used in peptide separation. The long-term objective of our research is to develop innovative approaches for the isolation of peptides at large scale. More specifically, our work will focus at developing fundamental knowledge on structure-function characteristics of milk peptides in relation with their ability to form nanostructures that can be used as processing tools in peptide separation. The research program will be initiated using peptide b-LG f1-8 but it is expected that the work will lead to the discovery of other self-assembling peptides from whey proteins. This original approach is likely to open new possibilities for the separation of minor compounds such as bioactive peptides from milk. The research program will provide unique opportunities to train highly qualified personnel (HQP) who will possess a strong background in milk proteins and their processing into hydrolysates and bioactive peptides. This research has a very high potential to generate innovations that will be useful for the Canadian dairy industry.

牛奶和乳清蛋白酶解产物已被广泛用作生物活性肽（例如抗高血压、免疫调节等）的来源。然而，开发有效的提取物需要将生物活性分子从这些水解产物中浓缩或甚至分离。我们最近的工作集中在来自β-乳球蛋白（b-LG）的肽片段，即b-LG f1-8，其在通过反渗透浓缩b-LG的胰蛋白酶水解产物后从聚集的材料中分离。发现该肽序列具有自组装特性，其导致在特定浓度、pH和温度值下形成纳米纤维和水凝胶。在初步实验中，我们观察到蛋白质水解物与肽b-LG f1-8的纳米纤维接触可以改变其肽组成，这表明这些纳米纤维可以在其表面吸附疏水性肽。拟议的工作是基于研究假设，这是可能的，从复杂的混合物中分离乳源性肽诱导自组装。在这些条件下预处理的肽混合物将表现出改善的物理化学稳定性，并且自组装肽又可用于肽分离。我们研究的长期目标是开发大规模分离肽的创新方法。更具体地说，我们的工作将集中在开发牛奶肽的结构-功能特性的基础知识，这些特性与它们形成纳米结构的能力有关，纳米结构可用作肽分离中的加工工具。该研究计划将使用肽b-LG f1-8启动，但预计这项工作将导致发现其他来自乳清蛋白的自组装肽。这种原始方法可能为从牛奶中分离生物活性肽等次要化合物开辟新的可能性。该研究计划将提供独特的机会，培养高素质的人才（HQP），他们将拥有牛奶蛋白及其加工成水解产物和生物活性肽的强大背景。这项研究有很大的潜力产生创新，将是有用的加拿大乳制品行业。