UV-visible Spectrophotometer

紫外可见分光光度计

• 批准号: 439258-2013
• 负责人: Bennet, Andrew
• 金额: $ 4.8万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=507070
• 关键词: UV; visible; Spectrophotometer

This proposal aims to solicit funds so that researchers at Simon Fraser University can replace a currently non-functional "UV-vis Spectrophotometer" with a top-quality research instrument. In order to understand chemical and biochemical reactions it is necessary to measure the rate of reaction, or how fast these processes occur. Ultra-violet and/or visible light is passed through a solution containing the compounds whose reaction we are studying and the spectrometer measures the changes in light as the reaction progress. This technique enables us to monitor processes that occur at rates between tens of seconds to several days. The investigators will use the equipment to answer fundamental question concerning relationships between: (i) the structure and function of proteins; (ii) the mechanisms of enzyme-catalyzed reactions; and (iii) questions concerning the speed at which biomolecules can react in the absence of enzymes. The results that are obtained by the investigators will have implications for numerous biological processes and disease states.

这项提议旨在筹集资金，使西蒙·弗雷泽大学的研究人员能够用一台顶级的研究仪器取代目前无法使用的“UV-VIS分光光度计”。为了了解化学反应和生化反应，有必要测量反应的速度，或这些过程发生的速度。紫外光和/或可见光穿过含有我们正在研究其反应的化合物的溶液，光谱仪测量光在反应过程中的变化。这项技术使我们能够监控以几十秒到几天的速度发生的过程。研究人员将使用该设备回答以下基本问题：(I)蛋白质的结构和功能；(Ii)酶催化反应的机制；(Iii)关于生物分子在没有酶的情况下反应速度的问题。研究人员获得的结果将对许多生物过程和疾病状态产生影响。