Catabolic genes and mobile elements of bacterial endophytes

细菌内生菌的分解代谢基因和移动元件

• 批准号: 170177-2013
• 负责人: Fulthorpe, Roberta
• 金额: $ 2.62万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=522762
• 关键词: Catabolic; genes; mobile; elements; bacterial

We depend on the bacteria of this world to dispose of our garbage. They do most of the work of transforming organic waste and toxic chemicals into the basic building blocks of carbon dioxide, water and mineral salts to be rebuilt into life forms and food. Bacteria live in all environments that have water. This includes the tissues of virtually all plants, where they live and obtain nutrition, and in some cases provide the plant with protection, growth stimulation and even toxin removal. The research proposed here addresses some basic questions about the bacteria within plants, known as bacterial endophytes, and their abilities to break down pollutants: How specific are endophytes to particular plant species? Does the plant host determine what organic compounds endophytes can break down? How important is the site that they grow in versus the species they are in? When in the plant environment, how much genetic transfer can occur between bacteria? We ask these questions by looking at the bacteria in plants from polluted and non-polluted sites. We also look at a particular species of bacteria, known to associate with plants, that carries importance toxin-degrading genes, and investigate how those genes are expressed and shared between bacteria.

我们依靠这个世界的细菌来处理我们的垃圾。它们完成了大部分的工作，将有机废物和有毒化学物质转化为二氧化碳、水和矿物盐的基本组成部分，然后再重建成生命形式和食物。细菌生活在所有有水的环境中。这包括几乎所有植物的组织，它们在那里生活和获得营养，并在某些情况下为植物提供保护，生长刺激甚至毒素去除。这里提出的研究解决了一些关于植物内细菌的基本问题，称为细菌内生菌，以及它们分解污染物的能力：内生菌对特定植物物种的特异性如何？植物宿主是否决定内生菌可以分解哪些有机化合物？它们生长的地点与它们所在的物种有多重要？在植物环境中，细菌之间可以发生多少遗传转移？我们通过观察污染和非污染地区植物中的细菌来提出这些问题。我们还研究了一种特定的细菌，已知与植物有关，携带重要的毒素降解基因，并研究这些基因如何在细菌之间表达和共享。