Development of a low cost solid-state nanopore fabrication method

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Nanopores are nanometer-scale holes embedded in thin insulating membranes. They provide a confined space within which single molecules can be captured and electrically interrogated at high throughput. Following 15 years of active research, nanopore-based sensors have emerged as the leading candidate for the next-generation of rapid, low-cost DNA sequencing technologies. The vision is that a nanopore would directly read the sequence of nucleotides from a strand of genomic DNA without any enzymatic amplifications, or use of labels. This achievement would place nanopore-based DNA sequencing as the cornerstone of personalized medicine, allowing for inexpensive (<$1,000), rapid (<24h) sequencing of an entire human genome. Nanopore-based technologies are also expected to have a significant impact as a tool used in disease diagnostic applications, including screening protein biomarkers, or profiling microRNA expression.