Surface Plasmon Resonance -Tilted Fibre Bragg Grating (SPR-TFBG) fibre optic biosensor to detect metastasized cancer cells in cancer patients

表面等离子共振-倾斜光纤布拉格光栅 (SPR-TFBG) 光纤生物传感器可检测癌症患者中的转移癌细胞

• 批准号: 453689-2013
• 负责人: Willmore, William
• 金额: $ 1.82万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=541648
• 关键词: Surface; Plasmon; Resonance; Tilted; Fibre

Spartan Bioscience Inc. is a leader in point-of-care DNA testing. They were the first to complete sample-to-result DNA testing system in medicine and have the Spartan RX and DX-12 DNA testing instruments currently on the market. Spartan is now currently interested in developing new technologies for the detection of metastasized tumor cells in cancer patients. The detection of cancer metastasis is an ongoing problem when utilizing the current technologies. Recently, a group at Carleton have developed a fibre optic biosensor that relies on Surface Plasmon Resonance (SPR) to detect the attachment of biomolecules, and even whole cells, onto the fibre's surface. This SPR device relies on a Tilted Fibre Bragg Grating (TFBG), imprinted into the fibre's core, to reflect light from the surface of the fibre and back down the fibre to a detector. Attachment events at the surface of the fibre are detected as changes in the SPR signal, which can include attachment/deattachment of cells to the fibre. The surface of the fibre can be functionalized to make it more cancer-specific with the attachment of DNA aptamers that recognize, and bind to, specific proteins uniquely expressed at the surface of the cancer cell. Spartan Bioscience has encountered challenges in developing such technology in that it does not have the in-house capabilities to a) manufacture such SPR-TFBG fibres, b) functionalize the surface of such fibres with DNA aptamers, or c) test them in cell culture for cancer versus non-cancer binding of cells. The company also does not have the analytical tools or the research personnel to solve this problem. The NSERC ENGAGE grant will be utilized as proof-of- principle testing of the fibre optic biosensor to a) attach DNA aptamers against mucin-1, a breast cancer marker, to the fibre itself and b) test for the attachment of a breast cancer cell (MCF7) versus non-cancer cell (MCF10). This endeavor will represent a new collaboration between Spartan Bioscience and our lab and will involve a multidisciplinary team of researchers (engineers, chemists and biochemists) to solve the problem. The duration of this project is expected to be 6 months and will serve as a basis for applications for a CIHR/NSERC CHRP grant.

斯巴达生物科学公司是即时DNA检测领域的领导者他们是第一个在医学上完成样本到结果DNA测试系统的公司，目前市场上有Spartan RX和DX-12 DNA测试仪器。Spartan目前对开发用于检测癌症患者转移肿瘤细胞的新技术感兴趣。当利用当前技术时，癌症转移的检测是一个持续的问题。最近，Carleton的一个小组开发了一种光纤生物传感器，该传感器依赖于表面等离子体共振（SPR）来检测生物分子甚至整个细胞在纤维表面上的附着。这种SPR设备依赖于一个倾斜的光纤布拉格光栅（TFBG），印在光纤的核心，以反射来自光纤表面的光，并沿着光纤返回到检测器。纤维表面的附着事件被检测为SPR信号的变化，其可以包括细胞与纤维的附着/脱附着。纤维的表面可以被功能化以使其更具有癌症特异性，并附着识别并结合在癌细胞表面独特表达的特定蛋白质的DNA适体。Spartan Bioscience在开发这种技术时遇到了挑战，因为它没有内部能力a）制造这种SPR-TFBG纤维，B）用DNA适体功能化这种纤维的表面，或c）在细胞培养物中测试它们的癌症与非癌症细胞结合。该公司也没有分析工具或研究人员来解决这个问题。NSERC ENGAGE赠款将用作光纤生物传感器的原理验证测试，以a）将针对粘蛋白-1（乳腺癌标记物）的DNA适体附着到光纤本身，以及B）测试乳腺癌细胞（MCF 7）与非癌细胞（MCF 10）的附着。这一奋进将代表Spartan Bioscience和我们实验室之间的新合作，并将涉及多学科研究人员（工程师，化学家和生物化学家）团队来解决这个问题。该项目的持续时间预计为6个月，并将作为申请CIHR/NSERC CHRP赠款的基础。