Transcriptome Analyses of Standing Genetic Variation and Phenotypic Plasticity in Conifer Trees

针叶树常备遗传变异和表型可塑性的转录组分析

• 批准号: 250069-2012
• 负责人: MacKay, John
• 金额: $ 3.42万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=547501
• 关键词: Transcriptome; Analyses; Standing; Genetic; Variation

Adaptability of plants and trees to accelerated climate warming and other environmental changes over the short term (decades to centuries) depends on their inherent phenotypic plasticity and standing genetic variation within existing populations. Recent studies show that a proportion of the genetic variants underlying complex phenotypes exert their effects through gene expression; so, discovering the genetic basis for the variation in transcript abundance is central to understanding phenotypic variation. My discovery research program aims to enhance our understanding of the conifer transcriptome as it relates to diversity and genetic architecture of complex traits. The proposal builds on my group's recent achievements including the development of large-scale RNA profiling capacity in conifer trees, discovery of extensive heritable variation in gene expression, discovery of transcriptional networks controlling metabolism, and an insect resistance gene. The short-term objectives presented in this proposal leverage transcriptome analyses to bridge between genetic diversity and phenotypes of adaptive significance in conifer trees, and aim to:

植物和树木在短期内(几十年到几百年)对加速的气候变暖和其他环境变化的适应性取决于它们固有的表型可塑性和现有种群中的长期遗传变异。最近的研究表明，部分复杂表型的遗传变异是通过基因表达来发挥作用的，因此，发现转录丰度变化的遗传基础是理解表型变异的核心。我的发现研究计划旨在加强我们对针叶树转录组的理解，因为它与复杂特征的多样性和遗传结构有关。这项建议建立在我的团队最近的成就基础上，包括在针叶树上发展大规模的RNA图谱能力，发现基因表达的广泛可遗传变异，发现控制新陈代谢的转录网络，以及抗虫基因。本提案提出的短期目标利用转录组分析在针叶树遗传多样性和具有适应意义的表型之间架起桥梁，并旨在：