Structure, genetic variability and regulation of the transcriptome in relation to wood formation in forest trees

林木木材形成相关转录组的结构、遗传变异和调控

• 批准号: 250069-2007
• 负责人: MacKay, John
• 金额: $ 3.79万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=455554
• 关键词: Structure; genetic; variability; regulation; transcriptome

Wood properties result from a genetically encoded program of development, known as secondary vascular growth or wood formation. Wood formation is a complex process because it is influenced by variations in environmental conditions over the growth season and the life of a tree. Gene expression is one of the mechanisms by which trees integrate genetic information and environmental cues in order to make wood. My program aims to identify and explore molecular links between genotypic variation and wood properties, through the study of genome-wide gene expression. The goal of my research is thus to provide insights into the genetic architecture of complex traits like wood. The transcriptome is comprised of the entire set of RNA transcripts present in a cell or tissue and represents the genes that may be expressed. We will use new microarrays to survey the expression of nearly all the genes present in the genomes of trees. The different subprojects will also rely on quantitative PCR and other methods to investigate the modulation and regulation of RNAs pools. Specific objectives are to (1) Develop reference profiles for conifer trees, comprised of diverse views of the dynamic transcriptome and provide a reference framework for our research and that of others; (2) Delineate the transcriptome modules that are linked to the processes underlying wood formation, as a function of daily and seasonal variation, spatial position in the tree and of environmental factors like drought; (3) Investigate the variability and genetic control of transcript abundance at the population level as a means to gain insights into the genetic architecture underlying complex traits; (4) Study mechanisms and networks underlying transcriptome regulation during wood formation in trees, through gain-of-function experiments with selected regulatory genes. The research will support the training of Ph. D. and M. Sc students. It will also result in applications in forestry, namely through the development of molecular breeding of forest trees.

木材的特性是由一个遗传编码的发育程序产生的，称为次生维管生长或木材形成。木材的形成是一个复杂的过程，因为它受到生长季节和树木生命中环境条件变化的影响。基因表达是树木整合遗传信息和环境信号以制造木材的机制之一。我的项目旨在通过全基因组基因表达的研究，识别和探索基因型变异和木材性能之间的分子联系。因此，我的研究目标是深入了解木材等复杂性状的遗传结构。转录组由存在于细胞或组织中的RNA转录物的整个集合组成，并且代表可以表达的基因。我们将使用新的微阵列来调查树木基因组中几乎所有基因的表达。不同的子项目还将依赖于定量PCR和其他方法来研究RNA库的调制和调节。具体目标是（1）开发针叶树的参考图谱，包括动态转录组的不同观点，并为我们的研究和其他人的研究提供参考框架;（2）描述与木材形成相关的过程的转录组模块，作为日常和季节变化、树木空间位置和干旱等环境因素的函数;（3）在群体水平上研究转录本丰度的变异性和遗传控制，作为深入了解复杂性状遗传结构的一种手段;（4）通过选择调控基因的功能获得实验，研究树木木材形成过程中转录组调控的机制和网络。本研究将为博士生培养提供有力的支持。和M.理科学生。它还将导致在林业中的应用，即通过发展林木的分子育种。