Characterization of complex soil and groundwater contamination scenarios using effect-directed analysis

使用效应导向分析表征复杂的土壤和地下水污染场景

• 批准号: 489004-2015
• 负责人: Hecker, Markus
• 金额: $ 1.71万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=586440
• 关键词: Characterization; complex; soil; groundwater; contamination

One of the main concerns associated with the risk assessment of legacy contaminant sites in Canada and globally is the complex and often un-documented historical release of chemicals resulting in unknown exposure patters and potential hazards across such sites. The main objective of this study is to apply and validate a novel bioassay-driven approach to characterize the complex contamination of soils and groundwater across a legacy pesticide manufacturing site owned by Interprovincial Cooperative Limited (IPCO). This approach is termed Effect-Directed-Analysis (EDA) and makes use of the properties of contaminants to interfere with specific biological processes. Specifically, soil and groundwater samples will be collected and subjected to analysis by an in vitro bioassay battery that determines general and specific toxic properties of a sample, including general toxicity (Microtox®), the presence of chemicals with genotoxic/mutagenic properties (Ames assay), dioxin-like compounds, acetylcholinesterase inhibitors (Ellman assay), and disruptors of steroidogenesis (H295R Steroidogenesis assay). These assays cover all general and specific toxicity pathways that would be expected to be associated with complex pesticide legacy contaminant sites and allow for broad non-target screening other unknown toxic constituents. Successful completion of the here-proposed bioassay-directed screen of soil and groundwater samples will provide IPCO with an initial data set that will allow for the identification of areas and matrices with elevated contaminant loads that may be of toxicological concern, and which will inform areas for future study.

与加拿大遗留污染场地风险评估有关的主要关切之一， 在全球范围内，化学品的历史释放是复杂的，往往没有记录， 这些场所的暴露模式和潜在危害。本研究的主要目的是应用和 验证一种新的生物测定驱动的方法，以表征土壤和地下水的复杂污染 在省际合作有限公司（IPCO）拥有的一个传统农药生产基地。这 这种方法被称为直接分析（EDA），它利用污染物的特性， 干扰特定的生物过程。具体而言，将收集土壤和地下水样本， 通过体外生物测定组进行分析，测定了 样品，包括一般毒性（Microtox®），是否存在具有遗传毒性/致突变特性的化学品 (Ames二恶英类化合物、乙酰胆碱酯酶抑制剂（Ellman试验）和 类固醇生成（H295 R类固醇生成测定）。这些试验涵盖所有一般和特定毒性途径 这将与复杂的农药遗留污染物场所有关，并允许广泛的 非靶向筛选其他未知毒性成分。成功完成此处提议的 对土壤和地下水样本进行生物测定指导的筛选将为IPCO提供初始数据集， 允许识别可能具有毒性的污染物负荷升高的区域和基质 这将为今后的研究提供信息。