G protein-coupled receptor interactome: Discovery and study of novel neighboring and interacting partners

该研究项目的重点是研究G蛋白偶联受体（GPCR）的相互作用组。其目的是确定和调查的作用，新的邻居和相互作用的合作伙伴参与贩运和下游信号级联的原型GPCR。为此，我们建议使用大肠杆菌生物素蛋白连接酶作为邻近依赖的识别策略。这种创新的，大规模的筛选方法应该导致发现新的合作伙伴和信号级联的GPCR在其“天然”的细胞环境。

This research program focusses on studying the interactome of G protein-coupled receptors (GPCRs). The aim is to identify and investigate the roles of novel neighboring and interacting partners involved in the trafficking and the downstream signaling cascades of prototypical GPCRs. To this end, we are proposing to use the Escherichia coli biotin protein ligase as a proximity-dependent identification strategy. This innovative, large scale screening approach should lead to the discovery of novel partners and signaling cascades of GPCRs in their “native” cellular environment.