Regulation of vascular responsiveness to changes in metabolic demand

调节血管对代谢需求变化的反应

• 批准号: RGPIN-2016-03698
• 负责人: Murias, Juan
• 金额: $ 1.82万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=614562
• 关键词: Regulation; vascular; responsiveness; changes; metabolic

Objectives of my research program The overall goal of my research program is to better understand how oxygen (O2) is transported and optimally distributed to the active tissues (skeletal muscles) to meet changes in O2 demand (i.e., moving from rest to higher intensities of exercise). My scientific approach I use exercise interventions to determine how effectively the vessels transporting O2 respond. Additionally, I study how aging and fitness status affect this transport of O2. For example, it is known that aging has negative effects on the O2 transport system (less responsive vessels). However, research has shown that highly trained people (even seniors) do not show a pronounced deterioration. Thus, I am interested in understanding whether the processes that affect these diminished responses are related to aging per se or to the lack of exercise that compromises the function of the vessels. My experimental approach includes non-invasive and minimally invasive studies using a human model. In this model, I use a number of non-invasive methodologies to examine O2 transport (blood flow) to the active muscles and vascular responsiveness (how the vessels react to distribute O2 to certain areas). By using innovative techniques that include measures of O2 consumption and O2 extraction in the muscles, as well as O2 delivery to the muscles, I obtain important information on how O2 is sent to the active parts of the body and how this response is affected by aging and fitness level, so that the processes that affect O2 delivery to the active tissues can be better understood. Additionally, the use of more invasive methodologies (i.e., muscle biopsies) allows me to examine changes at the cellular, molecular, and morphological level that have an impact on vascular control and O2 provision and distribution. Why is this important? My work provides new information (using novel methodologies and data analysis approaches) that can help us better understand how O2 is delivered to the tissues that need it, and what processes are involved in this adjustment. Combining the use of aging and fitness status models is important as these two factors are known to affect how the vessels respond and thus, they can provide us with more ways of learning about the factors that determine how the vessels make adjustments to deliver O2 to the right places. Increasing our knowledge in this area is very important to be able to provide adequate information on how the vessels function.

我的研究计划的目标 我的研究计划的总体目标是更好地了解氧气（O2）是如何运输和最佳分配到活动组织（骨骼肌），以满足O2需求的变化（即，从休息到更高强度的运动）。 我的科学方法 我使用运动干预来确定运输O2的船只如何有效地响应。此外，我还研究了衰老和健康状况如何影响O2的运输。例如，已知老化对O2运输系统具有负面影响（反应性较低的血管）。然而，研究表明，受过高度训练的人（甚至是老年人）不会表现出明显的恶化。因此，我有兴趣了解影响这些减弱的反应的过程是否与衰老本身有关，或者与缺乏锻炼而损害血管功能有关。我的实验方法包括使用人体模型的非侵入性和微创研究。 在这个模型中，我使用了一些非侵入性的方法来检查O2运输（血流）到活动肌肉和血管反应（血管如何反应以将O2分配到某些区域）。通过使用创新技术，包括测量肌肉中的O2消耗和O2提取，以及O2向肌肉的输送，我获得了有关O2如何被发送到身体活动部位以及这种反应如何受到衰老和健身水平影响的重要信息，以便更好地了解影响O2向活动组织输送的过程。此外，使用更具侵入性的方法（即，肌肉活组织检查）使我能够检查细胞、分子和形态学水平上的变化，这些变化对血管控制和O2供应和分布有影响。 为什么这很重要？ 我的工作提供了新的信息（使用新的方法和数据分析方法），可以帮助我们更好地了解O2是如何输送到需要它的组织的，以及这种调整涉及哪些过程。结合使用老化和健康状态模型很重要，因为这两个因素已知会影响血管的反应，因此，它们可以为我们提供更多的方法来了解决定血管如何调整以将O2输送到正确位置的因素。增加我们在这一领域的知识是非常重要的，能够提供足够的信息，血管如何运作。