Regulation of vascular responsiveness to changes in metabolic demand

调节血管对代谢需求变化的反应

• 批准号: RGPIN-2016-03698
• 负责人: Murias, Juan
• 金额: $ 1.82万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=660454
• 关键词: Regulation; vascular; responsiveness; changes; metabolic

Objectives of my research program***The overall goal of my research program is to better understand how oxygen (O2) is transported and optimally distributed to the active tissues (skeletal muscles) to meet changes in O2 demand (i.e., moving from rest to higher intensities of exercise). ******My scientific approach***I use exercise interventions to determine how effectively the vessels transporting O2 respond. Additionally, I study how aging and fitness status affect this transport of O2. For example, it is known that aging has negative effects on the O2 transport system (less responsive vessels). However, research has shown that highly trained people (even seniors) do not show a pronounced deterioration. Thus, I am interested in understanding whether the processes that affect these diminished responses are related to aging per se or to the lack of exercise that compromises the function of the vessels. My experimental approach includes non-invasive and minimally invasive studies using a human model.******In this model, I use a number of non-invasive methodologies to examine O2 transport (blood flow) to the active muscles and vascular responsiveness (how the vessels react to distribute O2 to certain areas). By using innovative techniques that include measures of O2 consumption and O2 extraction in the muscles, as well as O2 delivery to the muscles, I obtain important information on how O2 is sent to the active parts of the body and how this response is affected by aging and fitness level, so that the processes that affect O2 delivery to the active tissues can be better understood. Additionally, the use of more invasive methodologies (i.e., muscle biopsies) allows me to examine changes at the cellular, molecular, and morphological level that have an impact on vascular control and O2 provision and distribution.******Why is this important?***My work provides new information (using novel methodologies and data analysis approaches) that can help us better understand how O2 is delivered to the tissues that need it, and what processes are involved in this adjustment. Combining the use of aging and fitness status models is important as these two factors are known to affect how the vessels respond and thus, they can provide us with more ways of learning about the factors that determine how the vessels make adjustments to deliver O2 to the right places. Increasing our knowledge in this area is very important to be able to provide adequate information on how the vessels function.

我的研究计划的目标*我的研究计划的总体目标是更好地了解氧气(O2)是如何运输和优化分配到活跃组织(骨骼肌)的，以满足氧气需求的变化(即从休息状态转移到更高强度的运动)。*我的科学方法*我使用运动干预来确定输送氧气的血管的反应效率。此外，我还研究了衰老和健康状况如何影响氧气的运输。例如，众所周知，衰老对氧气运输系统(反应较慢的血管)有负面影响。然而，研究表明，训练有素的人(即使是老年人)的病情并没有明显恶化。因此，我有兴趣了解影响这些反应减弱的过程是与衰老本身有关，还是与缺乏损害血管功能的运动有关。我的实验方法包括使用人体模型的非侵入性和微创性研究。*在这个模型中，我使用许多非侵入性方法来检查氧气向活跃肌肉的运输(血液流动)和血管反应性(血管如何反应将氧气分配到特定区域)。通过使用创新的技术，包括测量肌肉中的氧气消耗和氧气提取，以及氧气向肌肉的输送，我获得了有关氧气如何被发送到身体活跃部位的重要信息，以及这种反应如何受到衰老和健康水平的影响，以便更好地了解影响氧气输送到活跃组织的过程。此外，使用更具侵入性的方法(即肌肉活检)使我能够检查细胞、分子和形态水平上的变化，这些变化对血管控制以及氧气的供应和分配产生影响。*为什么这一点很重要？*我的工作提供了新的信息(使用新的方法和数据分析方法)，可以帮助我们更好地了解氧气是如何被输送到需要它的组织的，以及这种调节涉及哪些过程。结合使用衰老和健康状态模型是重要的，因为这两个因素已知会影响血管的反应，因此，它们可以为我们提供更多的方法来了解决定血管如何进行调整以将氧气输送到正确位置的因素。增加我们在这一领域的知识是非常重要的，以便能够提供关于血管如何运作的充分信息。