QTRAP facility for trace metabolite detection and identification

用于痕量代谢物检测和鉴定的 QTRAP 设施

• 批准号: RTI-2017-00628
• 负责人: Harris, Cory
• 金额: $ 10.93万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616729
• 关键词: QTRAP; facility; trace; metabolite; detection

Funding is urgently required to replace an essential and heavily used 3200 QTRAP facility (now defunct) for detection of trace metabolites and differentiation of isobaric molecules at the U of Ottawa Core Facility for the Analysis of Natural and Synthetic Environmental Toxins (LANSET). Our researchers frequently need to find the “needle in the haystack” in complex biological or environmental samples, which may range from a toxin in soils, water or fish, to a phytochemical in plants, to a natural product or synthetic drug in animal tissues. In the past decade, the QTRAP facility has been essential to undertake challenging PhD research in our faculty and with many collaborating institutions. In the future the facility will be essential for for our funded programs such as ecotoxicology research on algal toxins in lakes, biomarkers of climate change in freshwater systems, and undesirable phytoestrogen effects in aquaculture, as well as natural product research on Canadian and tropical plants, their anxiolyic and metabolic bioactivities, and risk of pharmacokinetic interactions with drugs and toxins. The proposed 4000 QTRAP technology combines one of the highest selectivity methods of any detector with remarkable sensitivity at an affordable price with the full functionality of both triple quadrupole and ion-trap MS platforms. The QTRAP facility will allow current graduate students, PDFs and research associates to complete their research at an internationally competitive level. In addition, the facility provides essential support to collaborating external researchers and the private sector, including our own (Arnason) spin off company and multiple Canadian and international companies.

迫切需要资金，以取代一个重要的和大量使用的3200 QTRAP设施（现已停用）的痕量代谢物和同量异位分子的检测在U的渥太华核心设施的天然和合成环境毒素（LANSET）的分析。我们的研究人员经常需要在复杂的生物或环境样本中找到“大海捞针”，这些样本可能包括土壤、水或鱼中的毒素，植物中的植物化学物质，动物组织中的天然产物或合成药物。在过去的十年中，QTRAP设施已经在我们的教师和许多合作机构进行具有挑战性的博士研究至关重要。在未来，该设施将是必不可少的，为我们资助的计划，如生态毒理学研究藻类毒素在湖泊中，生物标志物的气候变化在淡水系统中，和不良的植物雌激素在水产养殖中的影响，以及天然产品的研究加拿大和热带植物，其抗焦虑和代谢的生物活性，药物和毒素的药代动力学相互作用的风险。拟议的4000 QTRAP技术结合了任何检测器中选择性最高的方法之一，具有卓越的灵敏度，价格合理，具有三重四极杆和离子阱MS平台的全部功能。QTRAP设施将允许当前的研究生，PDF和研究人员在国际竞争力水平上完成他们的研究。此外，该设施还为合作的外部研究人员和私营部门提供必要的支持，包括我们自己的（Arnason）分拆公司以及多家加拿大和国际公司。