Blow fly reproductive decisions impact on offspring development in the presence of anthropogenic and environmental stresses

在人为和环境压力存在的情况下，苍蝇繁殖决策对后代发育的影响

• 批准号: RGPIN-2017-05837
• 负责人: VanLaerhoven, Sherah
• 金额: $ 1.75万
• 依托单位: University of Windsor
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=631980
• 关键词: Blow; fly; reproductive; decisions; impact

Understanding the complexity and dynamics of interacting mechanisms remains one of the critical stumbling blocks in predictive applications of animal physiology, behaviour and ecology. This becomes even more critical when the predictive application in question involves estimates of the time of death of unattended human deaths using insect evidence. Insect evidence includes all developmental stages of insects that may be present or associated debris from these developmental stages, as well as any insect behaviour such as insect feeding and timing of adult arrival to the body, timing and location of egg laying on the body as these provide evidentiary value regarding the time frame or conditions of the body prior to discovery. My research focuses on insect reproductive and foraging decisions, and the outcomes of those decisions on the physiology and development of offspring under different abiotic and biotic stresses within natural and managed agroecosystems. I propose to evaluate how anthropogenic and biological mechanisms interact to change blow fly (Diptera: Calliphoridae) reproductive decisions and outcomes for offspring in dead animal based resource systems. Life history traits of blow flies, such as location and timing of egg laying and development of the resulting offspring, are used to provide these estimates of time of death. Despite the direct applicability of these biological metrics to issues of human importance, there are numerous untested assumptions in the literature that tend to be generalized across the family as a whole, such as the specific location that eggs are laid, timing of egg laying, numbers of eggs laid, and lower temperature thresholds for development. Of the 36 species in North America that utilize carrion, or dead animals, basic developmental data exists for less than 1/4 of these species. Even with the few better studied species, there is still debate as to lower temperature thresholds for development, timing of oviposition and limited information regarding other abiotic influences such as humidity or the effect of species interactions on these life history traits. Specifically, my research will measure timing and site selection of egg laying, number of eggs laid, survival, size and development of offspring of different species of blow flies at a range of temperatures, humidities and on different types of animal tissues. These life history traits will be measured for individual blow fly species on their own, as well as in combination with other blow fly species to measure the effect of species combinations on the expression of these traits. Finally, I will measure the distribution of different types of animal tissue sources (i.e. garbage, road kill) and this effect on blow fly species diversity and distribution. Together, this research will provide vital understanding of specific blow fly species life history traits which have direct applicability as insect evidence.

了解相互作用机制的复杂性和动态仍然是动物生理学，行为学和生态学预测应用的关键绊脚石之一。当所讨论的预测应用涉及使用昆虫证据估计无人看管的人类死亡时间时，这变得更加关键。昆虫证据包括可能存在的昆虫的所有发育阶段或这些发育阶段的相关碎片，以及任何昆虫行为，如昆虫进食和成虫到达尸体的时间，产卵在尸体上的时间和位置，因为这些对发现尸体前的时间框架或状况提供了证据价值。我的研究重点是昆虫生殖和觅食的决定，以及这些决定的结果对后代的生理和发育在不同的非生物和生物压力下的自然和管理的农业生态系统。我建议评估人类和生物机制如何相互作用，改变苍蝇（双翅目：丽蝇科）的生殖决策和结果的后代在死亡动物为基础的资源系统。苍蝇的生活史特征，如产卵的位置和时间以及由此产生的后代的发育，用于提供这些死亡时间的估计。尽管这些生物学指标直接适用于人类重要性的问题，但文献中有许多未经检验的假设倾向于在整个家庭中推广，例如产卵的具体位置，产卵的时间，产卵的数量以及发育的较低温度阈值。在北美36种利用腐肉或死亡动物的物种中，只有不到1/4的物种有基本的发育数据。即使有几个更好的研究物种，仍然有争议，以降低温度阈值的发展，产卵的时间和有限的信息，其他非生物的影响，如湿度或物种的相互作用对这些生活史特征。具体来说，我的研究将测量产卵的时间和地点选择，产卵的数量，不同种类的苍蝇在一系列温度，湿度和不同类型的动物组织中的后代的生存，大小和发育。这些生活史性状将单独测量个体蝇种，以及与其他蝇种组合测量物种组合对这些性状表达的影响。最后，我将测量不同类型的动物组织来源（即垃圾，道路杀戮）的分布，以及这种对苍蝇物种多样性和分布的影响。总之，这项研究将提供重要的了解特定的打击飞物种的生活史特征，有直接适用性的昆虫证据。