Multi-scale Investigations of the Environmental Transformation, Fate and Inhibitory Effects of Engineered Nanoparticles

工程纳米粒子（ENPs），如碳纳米管、银纳米粒子（nAg）和金属氧化物，现在可以在1000多种商业产品中找到。尽管纳米技术有许多预期的好处，但令人非常关切的是，我们尚未完全了解与这一革命相关的环境和健康风险。目前，加拿大没有有效的enp具体法规，环境和公共卫生保护机构正在争先恐后地收集必要的数据，以解决与纳米技术有关的安全和管理问题。美国国家研究委员会最近提出了一项关于ENP的环境、健康和安全（EH&S）方面的新研究策略，建议研究应侧重于最终控制ENP持久性、生物利用度、反应性和毒性的物理、化学和生物转化等关键因素。这些变化的复杂性和动态性使我们预测环境中ENPs释放相关风险的能力复杂化。基于我之前获得的NSERC发现基金的成果，这项研究计划的5年目标是确定常见的物理、化学和生物转化对土壤和水生环境中选定ENPs的转运、命运和抑制作用的影响。这项拟议的工作是第一个系统地研究不同的物理、化学和生物转化对ENP命运和相关长期暴露时间的影响的复杂影响。研究将集中在两种ENPs上，这两种ENPs存在于最多的商业产品中（nAg）或产量最大的产品中（nTiO2）。该研究计划的长期目标是为全球识别和量化与环境有害物质有关的环境和公共健康风险的努力作出有影响力的贡献。我们的研究将提供知识基础和资源，以追求在水系统中胶体现象的创新研究。显然，这项研究的潜在影响跨越了广泛的工业、环境和生物医学应用。科学方法将包括使用最先进的实验技术进行ENP聚集、溶解、运输和抑制/毒性的受控实验室研究，包括单粒子电感耦合等离子体质谱法、纳米颗粒跟踪分析、带有耗散监测的石英晶体微天平和带有增强暗场显微镜的高光谱成像。通过系统地改变关键环境条件和ENP特性（例如，尺寸，表面涂层化学，核心化学），我们将建立可测量参数与ehs风险评估目标端点之间的函数关系。为了将这些受控的实验室实验与自然条件联系起来，还将利用从加拿大地理位置收集的土壤和地下水进行研究。这项研究的ENPs具有精心控制但可变的表面功能化、尺寸和核心性质，将为支持全球环境风险评估工作做出重大的变革性贡献。这项研究的结果对于加拿大ENP生产商开发安全的ENP至关重要。拟议的研究计划优先考虑HQP的培训，使用综合多学科方法和协作培训环境。至少3名博士，3名硕士。， 5名本科生将被整合成一个拥有丰富现有经验的大型研究团队。通过该项目培训的学生将在加拿大工程和应用科学新兴领域的劳动力中具有重要价值。

Engineered nanoparticles (ENPs) such as carbon nanotubes, silver nanoparticles (nAg), and metal oxides can now be found in over 1000 commercial products. Despite the numerous anticipated benefits of nanotechnology, there is great concern that we do not yet fully understand the environmental and health risks associated with this revolution. Currently, there are no ENP-specific regulations in effect in Canada, and environmental and public health protection agencies are scrambling to gather the data required to address the safety and regulatory questions related to nanotechnology. The U.S. National Research Council recently proposed a new research strategy for environmental, health, and safety (EH&S) aspects of ENPs, recommending that research should focus on critical elements such as physical, chemical, and biological transformations that will ultimately control ENP persistence, bioavailability, reactivity, and toxicity. The complex and dynamic nature of these transformations complicates our ability to predict the risks associated with the release of ENPs in our environment. Building on the outcomes of my previous NSERC Discovery grant, the 5-year goal of the proposed research program is to establish the impacts of common physical, chemical, and biological transformations on the transport, fate and inhibitory effects of selected ENPs in soil and aquatic environments. The proposed work is the first to systematically examine the complex effects of different physical, chemical and biological transformations on ENP fate and impacts at relevant long-term exposure times. The research will focus on two of the ENPs that are found in largest number of commercial products (nAg) or produced in the largest quantities (nTiO2). The long-term objective of the research program is to make influential contributions to global efforts to identify and quantify the environmental and public health risks associated with ENPs. Our research will provide the knowledgebase and the resources to pursue innovative research on colloidal phenomena in aqueous systems. Clearly, the potential impact of the research spans over a broad range of industrial, environmental and biomedical applications. The scientific approach will include controlled laboratory studies of ENP aggregation, dissolution, transport and inhibition/toxicity using state-of-the-art experimental techniques, including single-particle-inductively coupled plasma mass spectrometry, nanoparticle tracking analysis, quartz crystal microbalance with dissipation monitoring, and hyperspectral imaging with enhanced darkfield microscopy. By systematically varying key environmental conditions and ENP properties (e.g., size, surface coating chemistry, core chemistry), we will develop functional relationships between measurable parameters and target end-points of EH&S risk assessment. To relate these controlled laboratory experiments to natural conditions, studies will also be conducted using soils and groundwater collected from Canadian geographical locations. This research with ENPs having carefully controlled but variable surface functionalization, sizes and core properties will make significant transformative contributions in supporting global environmental risk assessment efforts. The results of this research will be critical to Canadian ENP producers for the development of safe ENPs. The proposed research program prioritizes the training of HQP using an integrated multidisciplinary approach and a collaborative training environment. At least 3 Ph.D., 3 M.Eng., and 5 undergraduates will be integrated into a large research team where there is extensive existing experience. Students trained through this program will be of great value in the Canadian workforce in emerging areas of engineering and applied science.