Optimizing egg production for in vitro fertilization in zebrafish

优化斑马鱼体外受精的产卵量

• 批准号: 508796-2017
• 负责人: Benfey, Tillmann
• 金额: $ 1.57万
• 依托单位: University of New Brunswick
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=639328
• 关键词: Optimizing; egg; production; vitro; fertilization

Cryogenetics Services Inc. provides services and technology for short- and long-term storage of fish sperm forresearch, conservation and aquaculture applications, and they have an interest in applying this technology tozebrafish because of the growing importance of this model species for biomedical research. As an extension ofthis, they also wish to develop technologies for short- and long-term storage of zebrafish eggs and embryos. Tosupport their zebrafish program, Cryogenetics requires information on (1) the best rearing protocols to primeadult zebrafish for breeding, and (2) the best protocols for collecting and storing zebrafish eggs prior tofertilization. Having captive females produce high quality eggs at a predictable frequency requires optimizingtheir abiotic and biotic rearing conditions. The objectives of this project are therefore to develop protocols formaximizing egg production frequency and quality in zebrafish and to optimize the use of extender solution forin vitro fertilization. Research will focus on determining optimum adult feeding amount and frequency,between-spawning time interval, within-tank sex ratio, use of hormones to induce ovulation, andegg-to-extender ratio for storing eggs prior to fertilization.

低温遗传学服务公司该公司为研究、保护和水产养殖应用提供短期和长期储存鱼类精子的服务和技术，他们有兴趣将这一技术应用于斑马鱼，因为这一模式物种对生物医学研究越来越重要。作为这项研究的延伸，他们还希望开发出短期和长期储存斑马鱼卵和胚胎的技术。为了支持他们的斑马鱼计划，Cryogenetics需要以下信息：（1）最好的成年斑马鱼繁殖方案，以及（2）受精前收集和储存斑马鱼卵的最佳方案。让圈养的雌性以可预测的频率产生高质量的卵，需要优化它们的非生物和生物饲养条件。因此，本项目的目标是制定最大限度地提高斑马鱼卵子生产频率和质量的方案，并优化体外受精中使用的扩展液。研究将集中在确定最佳的成年喂养量和频率，产卵时间间隔，缸内性别比例，使用激素诱导排卵，以及受精前储存卵子的卵子与扩展剂的比例。