Optimizing egg production for in vitro fertilization in zebrafish

低温遗传服务公司为研究、养护和水产养殖应用提供短期和长期储存鱼类精子的服务和技术，他们有兴趣将这项技术应用于斑马鱼，因为这种模式物种对生物医学研究的重要性越来越大。作为这方面的延伸，他们还希望开发斑马鱼卵和胚胎的短期和长期储存技术。为了支持他们的斑马鱼计划，低温遗传公司需要以下信息：(1)用于繁殖的初级成年斑马鱼的最佳饲养方案，以及(2)在受精前收集和储存斑马鱼卵的最佳方案。要让圈养雌性以可预测的频率产生高质量的卵，需要优化它们的非生物和生物饲养条件。因此，该项目的目标是开发最大限度地提高斑马鱼产卵频率和质量的方案，并优化使用扩充剂溶液进行体外受精。研究重点将集中在确定最佳成虫摄食量和频率、产卵间隔时间间隔、水箱内性别比、激素诱导排卵的使用以及受精前储存卵子的G/E比。

Cryogenetics Services Inc. provides services and technology for short- and long-term storage of fish sperm forresearch, conservation and aquaculture applications, and they have an interest in applying this technology tozebrafish because of the growing importance of this model species for biomedical research. As an extension ofthis, they also wish to develop technologies for short- and long-term storage of zebrafish eggs and embryos. Tosupport their zebrafish program, Cryogenetics requires information on (1) the best rearing protocols to primeadult zebrafish for breeding, and (2) the best protocols for collecting and storing zebrafish eggs prior tofertilization. Having captive females produce high quality eggs at a predictable frequency requires optimizingtheir abiotic and biotic rearing conditions. The objectives of this project are therefore to develop protocols formaximizing egg production frequency and quality in zebrafish and to optimize the use of extender solution forin vitro fertilization. Research will focus on determining optimum adult feeding amount and frequency,between-spawning time interval, within-tank sex ratio, use of hormones to induce ovulation, andegg-to-extender ratio for storing eggs prior to fertilization.