Enhanced 2D Imaging for Gels and Membranes

增强凝胶和膜的二维成像

• 批准号: RTI-2018-00885
• 负责人: Audas, Timothy
• 金额: $ 10.73万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642166
• 关键词: Enhanced; 2D; Imaging; Gels; Membranes

The 2-dimensional imaging of biomolecules (i.e., DNA, RNA and protein) is a fundamental part of modern day molecular biology, biochemistry and chemistry, as a paper is seldom published in these fields that do not include data generated from the analysis of gels or membranes. Recent technological advances have markedly improved the resolution, sensitivity and multiplexing capabilities of these imagers, allowing for better quality and quantification of the data they acquire. Currently, the imaging resources available within the Faculty of Sciences at Simon Fraser University (SFU) are severely outdated and quickly reaching the end of their lifespan. Our gel documentation system has limited functionality (chemiluminescence detection only), and our only available biomolecular imager has recently suffered a permanent blue laser failure that has reduce our already limited (no near-infrared detection) spectral range. Together, this means that the research groups (>80 students from 25 labs) in our Faculty have insufficient multiplexing capabilities to satisfy increasingly rigorous scientific standards. This problem is exacerbated by a chronic oversubscription of users (>85 hours per month), making the acquisition of additional instrumentation a pressing concern for training HQP and future research planning.

生物分子(即DNA、RNA和蛋白质)的二维成像是现代分子生物学、生物化学和化学的基本组成部分，因为在这些领域发表的论文很少不包括凝胶或膜的分析产生的数据。最近的技术进步显著提高了这些成像仪的分辨率、灵敏度和多路复用能力，使它们获得的数据能够更好地质量和量化。目前，西蒙·弗雷泽大学(SFU)科学系内可用的成像资源严重过时，很快就会达到寿命的尽头。我们的凝胶记录系统功能有限(仅限化学发光检测)，而我们唯一可用的生物分子成像仪最近遭遇了永久性的蓝色激光故障，这缩小了我们已经有限的(近红外检测不到)光谱范围。总而言之，这意味着我们学院的研究小组(来自25个实验室的80名学生)没有足够的多路复用能力来满足日益严格的科学标准。用户长期超额订阅(每月85小时)加剧了这一问题，这使得购买额外的仪器设备成为培训HQP和未来研究规划的紧迫问题。