Integrating microbial characterization tools into conceptual site models (CSMs) at complex contaminated sites

该项目是 Geosyntec Consultants（安大略省圭尔夫）的水文地质学家和地球化学家与杜邦公司技术专家之间的密切合作，通过广泛的团队努力，更好地对大型复杂污染场地进行多方面的修复。由于广泛的地下水污染、地质异质性和长期的排放历史，这些地点的修复非常困难。其他非技术因素，包括社区影响、场地可访问性和法规遵从性，通常也会使清理工作变得复杂。该项目的总体目标是开发更好的方法来解决这些困难的地点。特别是，用于微生物群落分析的下一代 DNA 测序以及新的化合物特异性稳定同位素和**质谱分析将与从现场地下水井和土壤钻孔收集的传统数据相结合，以提高现场污染物归宿和迁移概念图的准确性。加拿大也不能幸免于拥有自己的一系列具有挑战性的污染场地。尽管本文提出的实际工作是使用巴西的一个场地作为测试案例，但该方法也同样适用于加拿大的复杂场地，例如萨尼亚工业园区或加拿大各地的其他化学精炼厂园区。该项目是真正的国际合作。拟议的研究将创建一个决策矩阵来指导补救实践，以更低的成本获得更好的结果。加拿大在场地修复方面已经拥有相当多的专业知识，并且世界各地都在寻求这种专业知识。该提案进一步增强了加拿大的声誉，并为所有参与的总部人员提供了独特的实践培训机会。**

This project is a very close collaboration between hydrogeologists and geochemists at Geosyntec Consultants (Guelph, ON) and technical experts at DuPont on an extensive team effort to better approach the multifaceted remediation of a large complex contaminated site. Remediation of such sites is difficult owing to extensive groundwater contamination, heterogeneous geology and a long history of releases. Other non-technical factors including community impacts, site accessibility, and regulatory compliance often also complicate the cleanup. The overall goal of this project is to develop better approaches to tackle such difficult sites. In particular, next generation DNA sequencing for microbial community analysis and new compound-specific stable isotope and**mass spectroscopy analyses will be integrated with traditional data collected from groundwater wells and soil borings at the site to improve the accuracy of the conceptual map of contaminant fate and transport at the site. Canada is not at all immune to having its own suite of challenging contaminated sites. Although the actual work proposed herein is using a site in Brazil as the test case, the approach will be equally applicable at complex sites here in Canada too, such as the Sarnia Industrial complex, or other chemical refinery complexes across Canada. The project is truly an international collaboration. The research proposed will create a decision**matrix to guide remediation practices for better outcomes at lower cost. Canada already has considerable expertise in site remediation and that expertise is sought around the world. This proposal further strengthens Canada's reputation and provides a unique opportunity for hands-on training for all HQP involved.**