Integrating microbial characterization tools into conceptual site models (CSMs) at complex contaminated sites

该项目是Geosyntec Consultants（圭尔夫，ON）的水文地质学家和地球化学家与杜邦公司的技术专家之间非常密切的合作，他们进行了广泛的团队努力，以更好地解决大型复杂污染场地的多方面修复问题。由于地下水污染范围广、地质不均匀和排放历史悠久，对这些地点进行补救是困难的。其他非技术因素，包括社区影响、网站可访问性和法规遵从性，通常也会使清理工作复杂化。该项目的总体目标是制定更好的方法来处理这些困难的网站。特别是，用于微生物群落分析的下一代DNA测序和新的化合物特异性稳定同位素和 ** 质谱分析将与从该场址地下水威尔斯井和土壤钻孔收集的传统数据相结合，以提高该场址污染物归宿和迁移概念图的准确性。加拿大完全不能幸免于拥有自己的一套具有挑战性的污染场地。虽然本文提出的实际工作是使用巴西的一个地点作为测试案例，但这种方法同样适用于加拿大的复杂地点，如萨尼亚工业综合体或加拿大各地的其他化学炼油厂。这是一个真正的国际合作项目。拟议的研究将创建一个决策矩阵，以指导补救做法，以更低的成本取得更好的结果。加拿大在场地补救方面已经拥有相当多的专门知识，世界各地都在寻求这种专门知识。这一提议进一步加强了加拿大的声誉，并为所有相关的HQP提供了一个独特的实践培训机会。**

This project is a very close collaboration between hydrogeologists and geochemists at Geosyntec Consultants (Guelph, ON) and technical experts at DuPont on an extensive team effort to better approach the multifaceted remediation of a large complex contaminated site. Remediation of such sites is difficult owing to extensive groundwater contamination, heterogeneous geology and a long history of releases. Other non-technical factors including community impacts, site accessibility, and regulatory compliance often also complicate the cleanup. The overall goal of this project is to develop better approaches to tackle such difficult sites. In particular, next generation DNA sequencing for microbial community analysis and new compound-specific stable isotope and**mass spectroscopy analyses will be integrated with traditional data collected from groundwater wells and soil borings at the site to improve the accuracy of the conceptual map of contaminant fate and transport at the site. Canada is not at all immune to having its own suite of challenging contaminated sites. Although the actual work proposed herein is using a site in Brazil as the test case, the approach will be equally applicable at complex sites here in Canada too, such as the Sarnia Industrial complex, or other chemical refinery complexes across Canada. The project is truly an international collaboration. The research proposed will create a decision**matrix to guide remediation practices for better outcomes at lower cost. Canada already has considerable expertise in site remediation and that expertise is sought around the world. This proposal further strengthens Canada's reputation and provides a unique opportunity for hands-on training for all HQP involved.**