High-throughput microfluidic technology for developing small 3D tissues**

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Conventional cell-based assays offer the opportunity to investigate cell-cell, cell-tissue, and cell-drug interactions in two-dimensional (2D) culture systems. These assays are used extensively to perform screening a library of molecules that modulate a certain target among a broad range of biological events in tissues. However, the cost of phenotypic cell-based screening using conventional 2D models is prohibitive for many companies for large-scale screening of molecules. Also, the existing 3D tissue models do not still recapitulate basic requirements of a real 3D tissue such as the interaction of cells with the 3D matrix under controlled perfusion system. In this project, we will develop a new technology for generating realistic 3D tissues with much smaller size, useful to make effective biomolecules for engineering of cells or treatment of tissues. Such technology enables making more realistic tissues with mimetic cell-cell, cell-tissue, and cell-biomolecules interactions, and helps to make small tissues interacting with a very small volume of biomolecules in a high-throughput system. This 3D tissue model will drastically reduce the cost of testing biomolecules performance, making the large-scale screening of target molecules affordable. This work will also eliminate the challenge of cross-contamination among small tissues, something that is a critical challenge in all existing 3D tissue assays. These 3D tissue models will benefit SV ChemBiotech in developing cost-effective tissue models and drastically reducing the cost of testing biomolecules performance, providing the access to a new market for the company via making the large-scale screening of target molecules affordable.****