Optical Coherence Tomography, Molecular Guided Imaging, and Surgical Navigation for targeted and controlled Laser Ablation

*目标*我们的广泛目标包括：*1)开发用于组织检测和勾画的新成像方法*2)用于显示和手术导航的成像方法的融合*3)用于治疗靶向的图像引导和融合*4)用于监测疗效的成像方法*具体目标是：**1)结构和血管(斑点方差和多普勒光学相干断层扫描)方面的持续工作；SV/D-OCT)用于检测和描绘血管系统的方法，以及采用这些用于功能成像的方法*2)继续发展用于OCT的弹性成像方法以描绘各种组织类型，包括脑组织结构和健康与患病组织*3)继续发展分子荧光技术，重点是采用定量的、深度分辨的、和3D方法*4)OCT和荧光数据与现有MVIGS系统融合*5)优化信息工作流程，防止认知过载*6)将导航信息转换为机器人安装的消融激光，用于对具有OCT控制的建筑物进行靶向和受控消融*技术重点、方法、迄今的工作*将继续开发BBL以前建立的核心技术，特别是：*1)OCT：一种基于红外的干涉方法，横向分辨率为10微米，深度成像可达3毫米。我们继续开发结构和血管成像方法(SV/D-OCT)以及OCE，并将继续将这些方法转化为外科设备。*2)分子引导荧光成像：该方法使用特定的分子荧光团对目标进行成像。我们已经开发了一种时间控制的生物光子学手术室，允许在不中断工作流程的情况下进行实时成像，并正在努力建立定量方法。*3)机器视觉图像制导手术(MVIGS)：我们开发了一种将结构光与术前成像相结合的导航系统，用于工具定位。我们的目标是继续研究开发将其他成像模式与我们当前的导航系统集成的方法，以优化工作流程。*4)制导激光消融：我们已经开发出在类似于笔记本电脑的尺寸包络中使用光纤激光进行OCT控制激光消融的方法。我们目前正在努力描述这些系统的特征，并将MVIGS与机器人一起用于宏观制导，将OCT和荧光用于微观制导。*IMPACT*上述研究对于将这些新技术转化为OR具有特别重要的意义。我们的目标是在考虑到工作流程的情况下将这些技术引入OR，并将它们的使用从神经外科手术室扩展到外科和医学的其他领域，并提供创造知识产权和创造就业的机会。**

This proposal from PI Yang continues work in the development of image-guided techniques for the neurosurgical operating room (OR) and beyond.***Objectives***Our broad goals include:***1) Development of novel imaging methods for tissue detection and delineation***2) Fusion of imaging methods for display and surgical navigation***3) Image-guidance and fusion for therapeutic targeting***4) Imaging methods for monitoring of therapeutic effects***Specific aims are:***1) Continued work on structural and vascular (Speckle Variance and Doppler Optical Coherence Tomography; SV/D-OCT) methods for the detection and delineation of vasculature, with adoption of these methods for functional imaging***2) Continued development of elastography methods for OCT for the delineation of various tissue types, including brain tissue structures and healthy vs. diseased tissues***3) Continued development of molecular fluorescence techniques, with focus on the adoption of quantitative, depth resolved, and 3D methods***4) Fusion of data for OCT and fluorescence with current MVIGS system***5) Optimization of information workflow to prevent cognitive overload***6) Translation of navigation information to robot-mounted ablation lasers for targeted and controlled ablation of structures with OCT control***Technological foci, methods, work to date***Core technologies previously established in by the BBL will continue to be developed, specifically:***1) OCT: An infrared based interferometric method with 10 micron lateral resolution, and depth imaging of up to 3mm. We have continued to develop methods for structural and vascular imaging (SV/D-OCT), as well as OCE, and will continue to translate these to a surgical setting.***2) Molecular guided fluorescence imaging: This method uses specific molecular fluorophores to allow imaging of targets. We have developed a temporally controlled biophotonics operating room allowing for real-time imaging without disruption of workflow, and are working to establish quantitative methods.***3) Machine Vision Image Guided Surgery (MVIGS): We have developed a navigation system that combines structured light with presurgical imaging for tool localization. We aim to continue to look at developing methods for the integration of other imaging modalities with our current navigation system for optimized workflow.***4) Guided Laser Ablation: We have developed methods for OCT-controlled laser ablation using fiber lasers in a size-envelope similar to that of a laptop. We are currently working to characterize these systems, and to use MVIGS with robotics for macroscopic guidance, and OCT and fluorescence for micro-guidance.***Impact***The above research is of particular importance to allow for the translation of these novel technologies into the OR. We aim to bring these technologies into the OR with workflow considerations in mind, and to expand their use beyond the neurosurgical operating room to other areas of surgery and medicine, with opportunity for IP generation and job creation.**