Histology core equipment

组织学核心设备

• 批准号: RTI-2020-00120
• 负责人: Richard, Caroline
• 金额: $ 9.24万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693405
• 关键词: Histology; core; equipment

This application is to update our histology core equipment (processing, embedding and microtome stations). The existing equipment is over 10 years old, have been repaired numerous times and is obsolete. The equipment is also not safe for the users or the environment as the space does not have sufficient ventilation to accommodate the processor which should be placed within a fumehood. This creates delays in tissue processing and students' project and adds additional cost that our discovery grants cannot cover. We would like to buy a free standing carbon filter enclosed system that will protect both the users and the environment for processing tissues along with the embedding station since the one we have does not cool samples anymore and we have to have an additional cold plate for blocking tissue. We also want to update the manual microtome to a semi-automated. The upgrade is not only more user friendly but would allow us to have more consistent tissue sectioning and it has additional safety features that protect the users.******An important novel aspect of Dr. Richard's Discovery grant is to understand the connection between the gut morphology, the gut-associated immune system and the peripheral or systemic immune system in the Wistar rat model of obesity. This requires to do immunohistochemistry on different sections of the gut. Dr. Jacobs' Discovery grant strives to understand the role of dietary choline, and related nutrients, on mammalian physiology. The importance of histological analysis cannot be overstated within these programs. The current equipment we are using has been repaired numerous times in the last year and parts for the repairs are not always available delaying protocol optimization and the training of HQPs. This justifies the need and urgency of replacing it now to prevent further delay. Many other collaborators, professors and their students in the Department use the histology core equipment. Since the histology core has multiple users, the Department provides a technician to operate and maintain the equipment on a daily basis which makes it more affordable to use. She also trains students and other HQPs on how to use the equipment. ******We have developed a program where trainees are exposed to all aspects of research, from basic science to translational applications i.e. “from test-tube to animal models to humans” using a variety of experimental approaches that include biochemical, cell biological, molecular biological, and in vivo techniques. This allows our HQP to have many “tools' in their professional “tool kit” that will improve their employability. Trainees who learn how to use histology equipment and how to analyze the results gain valuable skills that are an asset in many different careers (industry, healthcare, academia etc.). Each year, we train a number of HQPs that span from research associate, new lab technicians, graduate students as well as undergraduate students from different Department. **

这项应用是为了更新我们的组织学核心设备(加工、包埋和切片机)。现有的设备已有10多年的历史，经过多次维修，已经过时。该设备对使用者或环境也不安全，因为该空间没有足够的通风来容纳应放置在通风柜内的处理机。这造成了组织处理和学生项目的延迟，并增加了我们的发现助学金无法支付的额外费用。我们想购买一个独立式碳过滤器封闭式系统，它将保护用户和处理组织的环境以及嵌入站，因为我们有一个不再冷却样品，我们必须有一个额外的冷板来阻止组织。我们还想把手动切片机更新为半自动的。这一升级不仅对用户更加友好，还将允许我们进行更一致的组织切片，并具有保护用户的额外安全功能。理查德博士的发现基金的一个重要新方面是了解肥胖Wistar大鼠模型的肠道形态、肠道相关免疫系统和外周或全身免疫系统之间的联系。这需要在肠道的不同部分进行免疫组织化学。雅各布斯博士的发现基金致力于了解饮食中胆碱和相关营养素在哺乳动物生理学中的作用。在这些项目中，组织学分析的重要性怎么强调都不为过。我们目前使用的设备在过去一年中进行了多次维修，维修的部件并不总是可用的，延误了方案优化和HQP的培训。这证明现在有必要和紧迫地更换它，以防止进一步拖延。许多其他合作者、教授和他们的学生使用组织学核心设备。由于组织学核心有多个用户，该部提供了一名技术员每天操作和维护设备，使其更易于使用。她还培训学生和其他HQP如何使用这些设备。*我们开发了一个项目，学员可以接触到从基础科学到翻译应用的方方面面的研究，即使用包括生化、细胞生物学、分子生物学和活体技术在内的各种实验方法，即从试管到动物模型再到人类。这让我们的HQP在他们的专业“工具包”中有许多“工具”，这将提高他们的就业能力。学习如何使用组织学设备和如何分析结果的受训者获得了宝贵的技能，这些技能是许多不同职业(工业、医疗保健、学术界等)的财富。我们每年培训一批HQP，包括研究助理、新来的实验室技术人员、研究生以及来自不同系的本科生。**