System for Ultra-high Speed Imaging of Sonochemical Phenomena in Biological Cells and Transmitting Medium during Ultra Fast Phase Transformation

生物细胞和超快相变传输介质中声化学现象的超高速成像系统

• 批准号: RTI-2020-00765
• 负责人: Packirisamy, Muthukumaran
• 金额: $ 10.19万
• 依托单位: Concordia University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693528
• 关键词: System; Ultra; Speed; Imaging; Sonochemical

Even though the Optical Bio Microsystems Laboratory of the applicant houses various state-of-the-art research facilities, there is a dire need for Ultra-High Speed Imaging Facility synchronized with HIFU acoustic source, suitable for both biological and engineering applications. Biological applications include study of sonochemistry interactions with cells including neurons and other cancer cells under various internalized environments such as nano particles and exosomes. Such a facility would also enhance ongoing research on developing cutting edge technologies in the area of Ultra Fast Phase Transformation (UFPT) of engineering materials as wells as sono interactions in cells. The supported research programs include but not limited to: 1) Micro particle synthesis in UFPT, 2) Selective nano particle synthesis, 3) Sonochemical Investigation of Neuromodulation using Focused Ultrasound. The Sonochemical Investigation of cells using Focused Ultrasound from Photron enables our groups to conduct research on the mentioned programs. The ultra-high speed FASTCAM SA-Z provides megapixel image resolution at frame rates up to 21,000 fps from its highly light sensitive image sensor (monochrome ISO 50,000) with 12bit dynamic range delivering the ultimate imaging performance. The FASTCAM SA-Z provides frame rates greater than 2 million fps at reduced image resolution and shutter speeds as short as 159 nanoseconds. Acquiring this equipment would not only facilitate the real time imaging of biological and material phenomena under acoustic interactions but would also sustain leading-edge research success, strengthen training goals, enhance quality of dissertations, and shorten graduation time. Our groups currently have more than 30 graduate students and 5 postdocs working on the research programs that will benefit from this facility. The collaborators that will use the facility are from Goodman Cancer Research Center and Head and Neck Surgery Department of McGill University, Biological and Engineering Departments of Concordia and Atlantic Cancer Research Institute at Moncton. In addition to conducting cutting edge research, the equipment enables the team to validate their modeling and simulations also. We believe that the collaboration of these labs in multidisciplinary research around this equipment will lead to break through understandings and practical products that will have huge market for industries. The collaborations between three research institutes around this equipment include diverse applications in acoustics, materials development, cellular biology, head and neck cancer treatment, neuronal biology and exosomal interactions. The proposed research programs will open up new methodologies for acousto-bio interactions and new material development for many applications. Housed in Optical-bio Microsystems Lab, the system will be open to on-site and other inter-institutional collaborations across Canada including McGill, UdeM, ETS and École Polytechnique.*****

尽管申请者的光学生物微系统实验室拥有各种最先进的研究设施，但迫切需要与HIFU声源同步的超高速成像设施，适合生物和工程应用。生物学应用包括研究在不同的内化环境下，如纳米粒子和外体，与细胞，包括神经元和其他癌细胞的声化学相互作用。这种设施还将加强在工程材料的超快相变(UFPT)领域以及细胞中的声纳相互作用领域开发尖端技术的正在进行的研究。支持的研究项目包括但不限于：1)超微粒子合成，2)选择性纳米粒子合成，3)利用聚焦超声进行神经调节的声化学研究。使用来自Photron的聚焦超声波对细胞进行的声化学研究使我们的团队能够对上述项目进行研究。超高速FASTCAM SA-Z采用12位动态范围的高感光图像传感器(单色ISO 50,000)，以高达21,000 fps的帧速率提供百万像素的图像分辨率，提供终极成像性能。FASTCAM SA-Z在降低的图像分辨率和短至159纳秒的快门速度下提供高于200万fps的帧速率。获得这一设备不仅有助于在声相互作用下对生物和物质现象进行实时成像，而且将保持前沿研究成果，强化培养目标，提高毕业论文质量，缩短毕业时间。我们的团队目前有30多名研究生和5名博士后，从事将从这一设施中受益的研究项目。将使用该设施的合作者来自古德曼癌症研究中心和麦吉尔大学的头颈外科系，康科迪亚的生物和工程系，以及蒙克顿的大西洋癌症研究所。除了进行尖端研究外，该设备还使团队能够验证他们的建模和模拟。我们相信，这些实验室围绕这一设备进行的多学科研究的合作将导致对行业具有巨大市场的理解和实用产品的突破。围绕该设备的三个研究机构之间的合作包括在声学、材料开发、细胞生物学、头颈癌治疗、神经生物学和胞外相互作用方面的各种应用。拟议的研究计划将为声学-生物相互作用和许多应用领域的新材料开发开辟新的方法。该系统设在光学-生物微系统实验室，将向加拿大各地的现场和其他机构间合作开放，包括McGill、UdeM、ETS和巴黎理工大学。*