Evaluation of the possible potential of essential oils present in emulsion or under vapour against Covid-19

评估乳液或蒸气中精油对抗 Covid-19 的可能潜力

• 批准号: 554809-2020
• 负责人: Lacroix, Monique
• 金额: $ 3.64万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705684
• 关键词: Evaluation; possible; potential; essential; oils

The essential oils (EOs) and their chemical constituents are known to be active against a wide range of viruses like herpes simplex virus type 1 (HSV 1) (Astani et al., 2010). The active compounds in essential oils are normally related to the presence of terpenes, especially monoterpenes and phenolic compounds which are the volatiles part in essential oils. Some reports on natural extracts and essential oils have demonstrated antiviral activity against coronaviruses to some extent in both emulsion phase and vapor phase (Mani et al., 2020). Since the new strain of coronavirus, Covid-19, is still not completely understood, it is not yet possible to find which EOs will offer the best level of protection. However, it is plausible to assume that some of the EOs and related terpenes are likely to offer a measurable level of defense in the same way that they do with other known viruses such as HSV 1. We have demonstrated in several studies that EOs in vapor or in solution can inhibit bacteria and fungus, and even virus i.e. norovirus (flavonoids from citrus extract) (Gobeil et al., 2020). The main objectives of the proposal are: (1) Evaluate the minimum inhibitory concentrations (MIC) and fractional inhibitory concentrations (checkerboard method) of EOs (FIC calculation: evaluation of the synergy between EOs) under emulsion form against Covid-19 using broth microdilution and checkerboard method; (2) Evaluate the MIC and FIC of EOs in vapor form against Covid-19 using a new broth volatilization checkerboard method; (3) Develop EOs formulation that could be applied under solution or vapor form on Covid-19. The results of the study will permit to develop anti Covid-19 formulations under the form of emulsion or for vapor application for diverse applications such as working or food surface or even for air decontamination.

已知精油（EO）及其化学成分对多种病毒如单纯疱疹病毒1型（HSV 1）具有活性（Astani et al.，2010年）。精油中的活性成分通常与萜烯类化合物有关，特别是单萜烯和酚类化合物，它们是精油中的挥发性部分。关于天然提取物和精油的一些报道已经证明了在乳液相和气相中都具有一定程度的抗冠状病毒的抗病毒活性（Mani等人，2020年）。由于新型冠状病毒Covid-19仍未完全被了解，因此尚不可能找到哪些EO将提供最佳保护水平。然而，可以合理地假设一些EO和相关的萜烯可能以与其他已知病毒（如HSV 1）相同的方式提供可测量的防御水平。我们已经在几项研究中证明，蒸气或溶液中的EO可以抑制细菌和真菌，甚至病毒，即诺如病毒（来自柑橘提取物的类黄酮）（Gobeil等人，2020年）。本研究的主要目的是：（1）评价最低抑菌浓度（MIC）和部分抑菌浓度（棋盘法）（FIC计算：评价EO之间的协同作用）使用肉汤微量稀释法和棋盘法在乳剂形式下对抗Covid-19;（2）使用新的肉汤挥发棋盘法评估挥发性有机化合物对COVID-19的MIC和FIC;（3）开发可在Covid-19上以溶液或蒸汽形式应用的EO制剂。该研究的结果将允许开发乳液形式的抗Covid-19制剂或用于各种应用的蒸汽应用，例如工作或食品表面，甚至用于空气净化。