Mass spectrometry to reveal the role of lipids in protein misfolding

质谱法揭示脂质在蛋白质错误折叠中的作用

• 批准号: DP170102033
• 负责人: Prof Tara Pukala
• 金额: $ 22.58万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170102033
• 关键词: Mass; spectrometry; reveal; role; lipids

This project aims to develop an integrated mass spectrometry-based approach to determine structures and dynamics of membrane associated proteins, and discover how lipids cause protein misfolding implicated in neurodegeneration. Lipid membranes may influence protein (mis)folding, aggregation and related toxicity. However, the complex and dynamic nature of the lipid-protein interactions involved means traditional methods cannot easily characterise them, and they are poorly understood at a molecular level. The project expects to develop tools to bridge a critical gap in structural biology, while understanding lipid-protein interactions could lead to new therapies. This will ultimately provide benefits such as reduced health costs and better health outcomes.

该项目旨在开发一种基于质谱的综合方法，以确定膜相关蛋白的结构和动力学，并发现脂质如何导致与神经退行性疾病有关的蛋白质错误折叠。脂质膜可能影响蛋白质的折叠、聚集和相关毒性。然而，所涉及的脂质-蛋白质相互作用的复杂性和动态性意味着传统方法不能轻易地将其解析，并且在分子水平上对它们的了解很少。该项目希望开发工具来弥合结构生物学中的关键差距，而了解脂质-蛋白质相互作用可能会导致新的疗法。这将最终带来好处，如降低医疗费用和改善健康结果。