High speed, area CMOS camera for phase stable imaging and Computational Adaptive Optics

用于相位稳定成像和计算自适应光学的高速面阵 CMOS 相机

• 批准号: RTI-2021-00780
• 负责人: Bizheva, Kostadinka
• 金额: $ 7.16万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718784
• 关键词: speed; area; CMOS; camera; phase

Optical methods that can image the structure and blood flow of biological tissue and probe its normal / abnormal function in-vivo, non-invasively and with cellular-level resolution, can be used clinically for real-time, non-contact optical (virtual) biopsy. As such, they have the potential to revolutionize the early diagnostics and to aid the treatment (surgical and non-surgical) of many diseases, from potentially blinding neurodegenerative retinal diseases to various types of cancer. Optical Coherence Tomography (OCT) is an imaging modality that can image in-vivo, non-invasively and simultaneously the cellular structure, blood flow and physiological function of biological tissue. However, the OCT images suffer from optical aberrations such as defocus, astigmatism and chromatic aberrations, which cause image distortions and blur. When applied to OCT images, an image processing algorithm named Computational Adaptive Optics (CAO), can compensate numerically these aberrations. Processing OCT images with CAO requires that the images are free of motion artefacts. Heart rate, breathing rate and involuntary muscle twitching which occur at frequencies of ~0.5Hz to ~3Hz, can introduce motion artefacts in the images if the OCT image acquisition rate is comparable to or slower than these motions. Current OCT systems use linear array cameras with frame rates < 200 fps, therefore the volumetric OCT imaging rate is < 0.2 Hz. Recently, our research group has developed OCT technology based on an ultrafast area camera (~2,500 fps) that can achieve volumetric imaging rate of ~2.5Hz which can suppress some, though not all motion artefacts and especially fast eye motion. Here, we propose to replace the old camera with a new one which offers a frame rate of 4,500 fps, sufficiently high to suppress effectively motion-induced image artefacts and therefore allow for processing of the OCT images with CAO. The new ultrafast camera provides optimal combination of pixel resolution (2560 x 1920), fast frame rate (4,500 fps) and low noise. The camera can be integrated easily into a fully operational OCT system already available in our research lab and the integration will require minimal hardware and software modifications of the current OCT system. As an integral part of the OCT system, the new camera will be utilized for a number of collaborative research projects focusing on fundamental and applied biomedical research of various diseases such as potentially blinding ocular pathologies (age related macular degeneration, diabetic retinopathy, glaucoma, etc.), neurodegenerative conditions (Alzheimer's, etc.) and various types of cancer. Students working on this research will benefit from research that is made more competitive through the addition of the new camera to the OCT system and the capabilities it will provide. It will enable their work to be published in high-impact factor journals such as “Nature Biophotonics” and Light Science and Applications”.

光学方法可以对生物组织的结构和血流进行成像，并以非侵入性和细胞水平的分辨率在体内探测其正常/异常功能，可用于临床实时、非接触式光学（虚拟）活检。因此，它们有可能彻底改变早期诊断并帮助治疗（手术和非手术）许多疾病，从潜在致盲的神经退行性视网膜疾病到各种类型的癌症。光学相干断层扫描（OCT）是一种可以在体内、非侵入性地同时对生物组织的细胞结构、血流和生理功能进行成像的成像方式。然而，OCT图像存在散焦、像散、色差等光学像差，导致图像失真和模糊。当应用于 OCT 图像时，一种名为计算自适应光学 (CAO) 的图像处理算法可以对这些像差进行数值补偿。 使用 CAO 处理 OCT 图像要求图像不含运动伪影。如果 OCT 图像采集速率与这些运动相当或慢，则以 ~0.5Hz 至 ~3Hz 的频率发生的心率、呼吸速率和不自主肌肉抽搐可能会在图像中引入运动伪影。目前的 OCT 系统使用帧速率 < 200 fps 的线性阵列相机，因此体积 OCT 成像速率 < 0.2 Hz。 最近，我们的研究小组开发了基于超快面阵相机（~2,500 fps）的 OCT 技术，该技术可以实现~2.5Hz 的体积成像速率，从而可以抑制一些（但不是全部）运动伪影，尤其是快速眼动。在这里，我们建议用帧速率为 4,500 fps 的新相机替换旧相机，该帧速率足以有效抑制运动引起的图像伪影，从而允许使用 CAO 处理 OCT 图像。新型超快相机提供了像素分辨率 (2560 x 1920)、快速帧速率 (4,500 fps) 和低噪声的最佳组合。该相机可以轻松集成到我们研究实验室已有的完全可操作的 OCT 系统中，并且集成需要对当前 OCT 系统进行最少的硬件和软件修改。 作为 OCT 系统的一个组成部分，这款新相机将用于一系列合作研究项目，重点关注各种疾病的基础和应用生物医学研究，例如潜在致盲的眼部病变（年龄相关性黄斑变性、糖尿病性视网膜病变、青光眼等）、神经退行性疾病（阿尔茨海默氏症等）和各种类型的癌症。 从事这项研究的学生将受益于通过在 OCT 系统中添加新相机及其提供的功能而变得更具竞争力的研究。这将使他们的工作能够发表在《自然生物光子学》和《光科学与应用》等高影响力期刊上。