Multimodality optical coherence tomography for non-invasive imaging of the structure, function and metabolism of biological tissue

多模态光学相干断层扫描对生物组织的结构、功能和代谢进行无创成像

• 批准号: 312037-2013
• 负责人: Bizheva, Kostadinka
• 金额: $ 2.33万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=645556
• 关键词: Multimodality; optical; coherence; tomography; non

Optical coherence tomography (OCT) is currently the most advanced, non-invasive, ophthalmic diagnostic method, that provides 3D images of the human eye in real time with cellular level resolution. OCT can also measure blood flow and physiological responses from neural tissue. Dr. Bizheva's research group was the first to demonstrate recently reproducible functional OCT (fOCT) recordings from visually stimulated retinas in living animals. This pioneering work sets the background for future studies, where fOCT can be developed as a non-invasive alternative to invasive methods such as electroretinography (ERG), for clinical evaluation of retinal function in patients with potentially blinding retinal diseases. Currently, fOCT is in its infancy and there are a number of unresolved technical issues, related to the system's spatial and temporal resolution, SNR, imaging probe designs, and the procedures for the extraction, display and correct interpretation of the fOCT data. Here we present a research program designed to develop further the fOCT technology. We propose to resolve the technical problems by: a) use of novel light sources and detectors; b) implementation of fast line- or en-face scanning; c) utilizing FPGA / GPU technology to improve the data collection rate; and d) developing novel image processing algorithms for efficient extraction of the functional information. We will test and validate the fOCT in animal models of retinal diseases. The outcome of the proposed research program will lead to a novel, clinically viable technology for early diagnostics of potentially blinding diseases, monitoring the effectiveness of drug or surgical therapies, and basic studies of the origins and stages of development of retinal diseases. FOCT could have a significant impact on the health and wellbeing of Canadians and the treatment of debilitating or life threatening diseases. The proposed program will be carried out by undergraduate, graduate, and post-doctoral level researchers. Funding is requested for their salaries, travel, materials, and costs associated with publishing results. Canada will directly benefit from the training of these researchers through the new technologies and medical applications they develop in the course of their training.****

光学相干断层扫描（OCT）是目前最先进的、非侵入性的眼科诊断方法，其以细胞水平分辨率真实的时间提供人眼的3D图像。OCT还可以测量来自神经组织的血流和生理反应。Bizheva博士的研究小组是第一个展示最近可重现的功能性OCT（fOCT）记录的活体动物视觉刺激视网膜。这一开创性工作为未来的研究奠定了基础，fOCT可以作为侵入性方法（如视网膜电图（ERG））的非侵入性替代方法，用于潜在致盲性视网膜疾病患者的视网膜功能临床评价。目前，fOCT处于起步阶段，存在许多未解决的技术问题，涉及系统的空间和时间分辨率、SNR、成像探头设计以及用于提取、显示和正确解释fOCT数据的程序。在这里，我们提出了一个研究计划，旨在进一步发展fOCT技术。我们建议通过以下方式解决技术问题：a）使用新的光源和检测器; B）实现快速线或面扫描; c）利用FPGA / GPU技术提高数据采集速率;以及d）开发新的图像处理算法以有效提取功能信息。我们将在视网膜疾病的动物模型中测试和验证fOCT。拟议的研究计划的结果将导致一种新的，临床上可行的技术，用于潜在致盲疾病的早期诊断，监测药物或手术治疗的有效性，以及视网膜疾病的起源和发展阶段的基础研究。FOCT可能对加拿大人的健康和福祉以及对衰弱或危及生命的疾病的治疗产生重大影响。拟议的计划将由本科生，研究生和博士后水平的研究人员进行。要求为他们的工资、差旅费、材料费以及与发表结果有关的费用提供资金。加拿大将通过这些研究人员在培训过程中开发的新技术和医学应用，直接受益于对他们的培训。