Cell culture suite

我们的研究团队由两名化学工程师（Amsden和Fitzpatrick）和两名机械和材料工程师（Ploeg和Rainbow）组成，正在申请资金购买Cytation 1混合细胞成像和多模式检测系统，II级生物安全柜和Ibidi灌注系统。需要这套设备来支持研究创建用于工程软结缔组织（如肌腱、韧带和椎间盘髓核）的细胞指导支架的策略，以及复杂环境下生物材料宿主反应的体外模型。Cytation 1是一种独特的仪器，结合了自动数字显微镜成像和数据分析以及传统的微孔板分析物检测。该仪器在女王大学并不存在，将为我们的团队和更广泛的女王大学研究工作提供关键的分析能力。作为阿姆斯登实验室的主力设备，老化失效的安全柜被要求更换为二级生物安全柜。由于目前女王大学校园内使用类似的灌注系统有限，因此迫切需要专用的Ibidi灌注系统来支持生物材料宿主反应的人体3D组织模型的开发。这些仪器的主要使用者将是申请人研究小组的研究生，博士后研究员和本科生研究人员。我们预计在未来5年内将有15到20名HQP接受使用该设备的培训。这些HQP将从事的项目旨在为新兴的加拿大生物医学和生物技术产业培训人员。此外，鉴于所需设备的多功能性，预计将有助于吸引新的HQP到我们在女王大学的研究项目中，并培养新的跨学科研究项目和合作。为了在这些项目中雇佣HQP，我们将实施EDI最佳实践，以确保我们的团队是多样化的。我们擅长解决独特的研究问题。此外，我们将优先考虑有效的沟通，并在团队合作中提倡公平的价值。这些做法将确保我们保持团队的多样性，并为所有合格的候选人提供机会。

Our research team, consisting of two Chemical engineers (Amsden and Fitzpatrick) and two Mechanical and Materials Engineers (Ploeg and Rainbow), are requesting funds to purchase a Cytation 1 hybrid cell imaging and multi-mode detection system, a level II biological safety cabinet, and an Ibidi perfusion system. This equipment suite is needed to support research into strategies to create cell instructive scaffolds for engineering soft connective tissues such as tendons and ligaments and the nucleus pulposus of the intervertebral disc, and an in vitro model of biomaterial host responses in complex environments. The Cytation 1 is a unique instrument that combines automated digital microscopy imaging and data analysis and conventional microplate analyte detection. This instrument does not exist at Queen's and would provide critical analytical capacity to our team and to the broader Queen's university research endeavour. A level II biological safety cabinet is requested to replace an aging and failing safety cabinet that is a workhorse in the Amsden lab. A dedicated Ibidi perfusion system is urgently needed to support the development of human 3D tissue models of biomaterial host responses as access to similar perfusion systems is currently limited on Queen's campus. The primary users of these requested instruments will be the graduate students, post-doctoral fellows, and undergraduate researchers in the applicants' research groups. We anticipate between 15 to 20 HQP will be trained using this equipment over the next 5 years. The projects these HQP will be working on are designed to train personnel for the emerging Canadian biomedical and biotechnology industries. Moreover, given the versatility of the requested equipment, it is expected to contribute to attracting new HQP to our research programs at Queen's as well as to cultivate new interdisciplinary research programs and collaborations. To hire HQP for these projects, we will implement EDI best practices to ensure our teams are diverse. and we excel at tackling unique research questions. In addition, we will prioritize effective communication, and promote the value of equity in teamwork. These practices will ensure that we maintain our group diversity and provide opportunities to all qualified candidates.