Cell culture suite

细胞培养套件

• 批准号: RTI-2022-00275
• 负责人: Amsden, Brian
• 金额: $ 9.63万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=741898
• 关键词: Cell; culture; suite

Our research team, consisting of two Chemical engineers (Amsden and Fitzpatrick) and two Mechanical and Materials Engineers (Ploeg and Rainbow), are requesting funds to purchase a Cytation 1 hybrid cell imaging and multi-mode detection system, a level II biological safety cabinet, and an Ibidi perfusion system. This equipment suite is needed to support research into strategies to create cell instructive scaffolds for engineering soft connective tissues such as tendons and ligaments and the nucleus pulposus of the intervertebral disc, and an in vitro model of biomaterial host responses in complex environments. The Cytation 1 is a unique instrument that combines automated digital microscopy imaging and data analysis and conventional microplate analyte detection. This instrument does not exist at Queen's and would provide critical analytical capacity to our team and to the broader Queen's university research endeavour. A level II biological safety cabinet is requested to replace an aging and failing safety cabinet that is a workhorse in the Amsden lab. A dedicated Ibidi perfusion system is urgently needed to support the development of human 3D tissue models of biomaterial host responses as access to similar perfusion systems is currently limited on Queen's campus. The primary users of these requested instruments will be the graduate students, post-doctoral fellows, and undergraduate researchers in the applicants' research groups. We anticipate between 15 to 20 HQP will be trained using this equipment over the next 5 years. The projects these HQP will be working on are designed to train personnel for the emerging Canadian biomedical and biotechnology industries. Moreover, given the versatility of the requested equipment, it is expected to contribute to attracting new HQP to our research programs at Queen's as well as to cultivate new interdisciplinary research programs and collaborations. To hire HQP for these projects, we will implement EDI best practices to ensure our teams are diverse. and we excel at tackling unique research questions. In addition, we will prioritize effective communication, and promote the value of equity in teamwork. These practices will ensure that we maintain our group diversity and provide opportunities to all qualified candidates.

我们的研究团队由两名化学工程师(Amsden和Fitzpatrick)和两名机械和材料工程师(Ploegg和Rainrow)组成，正在申请资金购买Cytation 1混合细胞成像和多模式检测系统、II级生物安全柜和Ibiti灌流系统。这一设备套件需要支持研究创造用于工程软结缔组织的细胞指导支架的策略，如肌腱、韧带和椎间盘髓核，以及复杂环境中生物材料宿主反应的体外模型。Cytation 1是一种独特的仪器，它结合了自动数字显微镜成像和数据分析以及传统的微孔板分析物检测。女王大学不存在这种仪器，它将为我们的团队和更广泛的女王大学研究工作提供关键的分析能力。需要一个二级生物安全柜来取代老化和故障的安全柜，后者是阿姆斯登实验室的主力。由于皇后园区目前对类似的灌流系统的使用有限，迫切需要一个专用的IBIDI灌流系统来支持生物材料宿主反应的人体3D组织模型的开发。这些申请仪器的主要用户将是申请者研究小组中的研究生、博士后研究员和本科生研究人员。我们预计在未来5年内，将有15到20名HQP使用这种设备进行培训。这些HQP将开展的项目旨在为加拿大新兴的生物医药和生物技术行业培训人才。此外，鉴于所需设备的多功能性，预计它将有助于将新的HQP吸引到我们在女王学院的研究项目中，并培养新的跨学科研究项目和合作。为了为这些项目聘请HQP，我们将实施EDI最佳实践，以确保我们的团队多样化。我们擅长处理独特的研究问题。此外，我们将把有效沟通放在首位，并促进团队合作中公平的价值。这些做法将确保我们保持集团的多样性，并为所有合格的候选人提供机会。