小麦籽粒储藏蛋白除受高、低分子量谷蛋白亚基基因和麦溶蛋白亚基基因控制外，还与小麦籽粒表达的转录因子调控有关。SPA（storage protein activator）是bZIP转录因子家族成员之一，在小麦籽粒形成过程中，参与了某些储藏蛋白基因的表达调控。研究SPA对小麦籽粒储藏蛋白基因的调控作用，可为小麦品质遗传改良提供分子生物学理论依据。 本研究以300份中国小麦种质资源、SPA表达量不同的小麦近等基因系(NILs)、SPA超表达和下调表达转基因后代株系为材料，于小麦灌浆期，每隔5天取小麦籽粒，用Real time PCR分析SPA及小麦籽粒储藏蛋白基因表达水平，研究SPA对小麦籽粒储藏蛋白基因的表达调控。以期探索在小麦籽粒发育过程中SPA对小麦储藏蛋白基因的调控机制。

Wheat storage protein is not only influenced by high molecular weight glutenin subunit , low molecular weight glutenin subunits and gliadin subunits genes,but also regulated by wheat transcriptional activator expressed in endosperm.SPA (storage ptotein activator) is a member of transcriptor bZIP family,and plays tanscriptional activator role in some wheat storage protein genes' expression.It will offer theortical basis for wheat quality improvement to study SPA's regulatory mechanism to storage protein genes. In this study, 300 wheat genetic resources from China, Near-isogenic lines (NILs)which different only in SPA expression level,SPA overexpression and downregulated transgenic lines will be used to study the regulation effect of SPA to wheat storage protein genes.Real time PCR will be conducted to detect SPA and wheat storage protein genes expression patterns during grain formation process.The aim of the study is to explore the regulatory mechanism of SPA to wheat storage protein genes.