小分子与核酸的相互识别和调控作用

本项目重点围绕与核酸相互作用的活性小分子的设计与合成，将合成的小分子作为探针用于核酸的识别和调控。核酸以其复杂多变的高级结构行使着多种调控功能, 通过不同方法和技术识别及调节核酸的二级结构是化学生物学的核心内容。 这个领域已经取得很多激动人心的进展, 但是目前仍然存在很多困难。 小分子由于其好的可修饰性与可控性, 在利用小分子对核酸的相互作用及功能调控方面研究将显现出了很好的优势, 研究小分子与核酸之间的相互作用，可以为我们探知核酸的生物医学功能， 也可以指导并加速抗疾病药物的发展，为人类战胜疾病提供理论基础和技术。为此，我们申请的项目将主要集中在下面几个方向上开展研究： I. 可诱导的核酸交联剂的设计、合成及生物活性研究；II. 小分子对四链核酸（主要针对RNA四链核酸）的识别和调控；III. 小分子对核酸的修饰和生物功能作用。

This proposal will focus on design of active small molecules, using as probes for nucleic acids recognition and regulation. Nucleic acids are the most significant macromolecules in living systems, whose complicated and variable secondary structures make them core elements in biofunction regulation. Dispite much progress, there still have challenges and room to improve. Small molecules could be as good candidates of nucleic acids probes, due to their good controllablity and modifiability. They could be used to detect and/or regulate certain targets and also could be developed as drugs. So, developing small molecule probes would be an important strategy for both theoretical and applied research. Herein, our project would include three major part: I. incudible cross-linking or alkylating agents for antitumor study; II. small molecules as probes for RNA G-quadruplex studies; III. small molecules as probes for nucleic acids epigenetic modification studies.