铅、镉等重金属已成为危害食品安全和人体健康的重要污染物。前期研究表明益生菌具有直接减除食品中重金属的潜力，同时也可缓解铅、镉暴露对机体造成的氧化应激和损伤。为深入理解益生菌生物减除重金属的分子机制，本课题以具有相关优良性状的植物乳杆菌CCFM8661及CCFM8610为研究对象，首先通过元素形态生化分析、代谢组学及比较基因组学手段研究阐明菌株在重金属暴露过程中生物响应机制；并利用蛋白质组学分析、分子生物学验证及固定金属离子亲和色谱手段研究菌株生物减除重金属的蛋白质分子机制；同时利用细胞和动物模型及转录组、宏基因组等组学手段，进一步研究菌株在肠道中对铅、镉吸收的抑制作用，以解析益生菌在宿主体内生物减除重金属的生理机制。

The contamination of heavy metals including lead (Pb) and cadmium (Cd) has become an important threat to food safety and human health. Our preliminary work showed that probiotics have the ability to remove heavy metals in food, as well as to alleviate Pb and Cd induced oxidative stress in animals. The objective of this study is to further understand the biological removal mechanism of probiotics including Lactobacillus plantarum CCFM8661 and CCFM8610. Firstly, the biological response of probiotics during the heavy metal exposure is investigated with element speciation, metabolomic and comparative genomic analysis. Additionally, the protein molecular mechanism of biological removal is evaluated by proteomic analysis, molecular biological confirmation and immobilized metal affinity chromatography methods. Moreover, cell and animal models are established and the effects of probiotics on the inhibition of heavy metal absorption in the gut are investigated with omics-based analysis such as transcriptomic and metagenomic methods, in order to demonstrate the biological removal mechanisms of probiotics in vivo.