毛白杨木材品质性状全基因组关联分析及关键基因的功能解析

如何全面、准确、高效地检测影响多年生植物种质资源木材品质的遗传调控位点并对其生物学功能进行验证是本项目拟解决的关键科学问题。本项目以毛白杨优异种质资源（528株个体）为材料，基于已完成的种质资源群体重测序数据，并联合利用群体转录组测序和单碱基编辑技术，系统开展：（1）毛白杨木材品质性状的全基因组关联分析以筛选关键遗传调控位点；（2）毛白杨木材品质性状遗传调控位点的转录调控网络构建；（3）联合单碱基编辑技术对毛白杨木材品质遗传调控位点的生物学功能进行解析。研究结果将揭示毛白杨优异种质资源木材品质性状遗传变异规律，阐明毛白杨种质资源木材品质关键遗传调控位点的遗传效应与转录调控机制，并重构毛白杨木材品质性状的关键遗传调控通路。项目的实施，将为多年生木本植物核心种质木材品质性状的评价与利用提供原创性成果，具有重要的科学价值。

Detection and dissection of genetic regulators of wood-property traits of perennial woody plants germplasm resources and verification their function, is important for complex traits analysis of germplasm resources of perennial woody plants. In our project, combining with the genome re-sequencing, RNA- sequencing data of Populus tomentosa and base editing technique, we use 528 individuals of germplasm populations from P. tomentosa to systematically launch several research topics as follows: 1) Genome wide association analysis of wood property traits; 2) Construction of transcriptional regulation network involved in wood property traits; 3) Biological function analysis of associated SNPs using base editing technique. Therefore, our study could reveal the genetic variation of wood-property traits of poplar, dissect the roles of wood properties associated SNPs on transcriptional regulation and their genetic effects, and reconstruct the genetic regulatory pathways related to wood quality of P. tomentosa. This work will provide innovative achievements on solving the significant theoretical and technical matters in protection and utilization of germplasm resources of perennial woody plants.