目前我国终末期肾脏病患者越来越多，肾脏移植是解决此问题最佳办法，但是存在肾源短缺以及移植后排斥问题。有研究显示以新生大鼠肾脏细胞对大鼠肾脏脱细胞支架进行再细胞化，可以培育出有功能可移植的肾脏。但从转化医学角度来看，只有培育出与人体肾脏大小、细胞种类及数目相近的大动物肾脏才能应用于临床。本研究以猪为研究对象，制作猪肾脱细胞支架，制备诱导多能干细胞（IPSCs）、分离培养肾脏成体干细胞及脐静脉内皮细胞对支架进行再细胞化，从细胞、分子及动物肾脏移植实验等方面探讨IPSCs及成体干细胞在肾脏再生中的作用并且研究大动物肾脏再生。本研究拟解决大动物肾脏去细胞化、再细胞化以及干细胞大量扩增等一系列技术，制备大动物有功能可移植的肾脏，为制备人体组织工程肾脏提供基础。

There are increasing numbers of people approaching and living with end-stage renal disease(ESRD) in our country. Two common treatments exist: dialysis to maintain life, and for more fortunate patients, transplantation of a functional kidney. There is, however, a serious shortage of transplantable kidneys.It is reported that a functional and transplantational kidney can be made by seeding human umbilical vein endothelial cells and rat neonatal kidney cells (NKCs).However，on the point of translational medicine, it's the only way to the clinical application by regenerating the engineered kidney of large animals. And the function of induced pluripotent stem cells（IPSCs） and adult kidney stem cells in the decellularized kidney scaffold is known less. To regenerate functional kidneys, we will seed porcine kidney scaffolds with porcine umbilical vein endothelial cells，adult kidney stem cells and IPSCs. We are going to detect the function of adult kidney stem cells and IPSCs in the scaffold using some methods, such as immunoblotting,immunofluorescence,flow Cytometry,etc. it's aimed to solve some technics such as decellularization , recellularization,etc. If we make some success, the problem of shortage of implantable kidney could be solved