A Hollow Fiber Bioreactor for the Production of Monoclonal Antibodies

采用径向流中空纤维哺乳动物细胞培养系统对非锚定依赖性小鼠杂交瘤进行连续培养。目前正在研究单克隆抗体的连续生产。采用杂交瘤细胞系PM-81和AML-2-23进行大规模培养和单克隆抗体生产研究。项目还进行了可行性评估；也就是说原位;反渗透膜分离抗体，并考察其培养和抗体产生的长期稳定性。分析是辅以数学模型，其中包括流体动力学，膜运输，和瞬态行为的培养系统。

A radial flow hollow fiber mammalian cell culture system is to be used for continuous culture of non-anchorage dependent murine hybridoma. Continuous production of monoclonal antibodies is being investigated. Hybridoma cell lines PM-81 and AML-2-23 are used for mass culture and monoclonal antibodies production studies. The project also evaluates the feasibility of ;itin situ;ro membrane separation of antibodies, and investigates the long-term stability of the culture and antibodies production. Analysis is complemented by mathematical models which incorporate hydrodynamics, membrane transport, and transient behavior of culture systems.