The Ultrastructural Correlates of Neural Transplantation and Development

斯科特博士将使用相关扫描传输 电子显微镜; 2）免疫细胞化学;和， 3)微血管造影来解决三个基本问题， 细胞相互作用的潜在机制， 发生在正常的产生神经肽的胎儿下丘脑 移植物和成年宿主大鼠的Brattleboro品系， 纯合子常染色体尿崩症 宿主老鼠的大脑 将检查存活的留置胎儿神经移植物， 确定神经元的神经解剖模式， 神经血管的整合，发展之间的胎儿 下丘脑移植和周围的宿主组织， 面对以下三个基本问题： 1)血管生成的潜在机制是什么？ 以及神经血管的相互关系 在宿主大脑和移植物之间， 外源性胎儿神经移植物的活性 2)这些细胞的超微结构相关性是什么 突触发生和神经可塑性的机制， 移植物和宿主 3)什么是精细结构相关和主要的 用于递送中枢作用的神经解剖途径 缩氨酸？ 从下面描述的这些实验设计中获得的数据可以 作为一个模型来建立一套精细的结构相关性 这可能适用于未来的神经移植。

Dr. Scott will employ 1) correlative scanning-transmission electron microscopy; 2) immunocytochemistry; and, 3) microangiography to address three basic questions that deal with the underlying mechanisms of cellular interactions that occur between normal neuropeptide-producing fetal hypothalamic transplants and adult host rats of the Brattleboro strain with homozygous autosomal diabetes insipidus. The brains of host rats with viable indwelling fetal neurografts will be examined to determine the neuroanatomical patterns of neuronal and neurovascular integration that develop between the fetal hypothalamic transplants and surrounding host tissue and will confront the three following basic questions: 1) What are the underlying mechanisms of formation of vascular patterns and neurovascular interrelationships that develop between the host brain and the graft which serve to maintain the viability of extrinsic fetal neurografts? 2) What are the ultrastructural correlates underlying the mechanisms of synptogenesis and neuronal plasticity between the transplant and the host? 3) What are the fine structural correlates and major neuroanatomical routes for the delivery of centrally acting peptides? Data garnered from such experiments designs depicted below can serve as a model to establish a set of fine structural correlates that may apply to future neural transplantation.