Molecular Systematics of Ribosomal RNA Genes in the Tricholomataceae

杜克大学的Rytas Vilgalys博士将使用现代DNA分析方法获得担子菌科毛霉科三个相关部落真菌的核苷酸序列数据和限制位点图谱信息。基因组数据将用于改善对这些真菌属群的识别，并确定属和部落之间的系统发育亲缘关系。将调查这些真菌的系统发育有用的限制性酶切位点和编码核糖体RNA的基因座的序列变异。此外，Vilgalys博士还改进了丝状真菌的实验室程序，使之能够快速、直接地对粗细胞裂解物中分离出的RNA进行测序。进一步的遗传分析将提供有关真菌核糖体基因内进化速度和方向的信息。

Dr. Rytas Vilgalys of Duke University will use modern methods of DNA analysis to obtain nucleotide sequence data and restriction-site mapping information on fungi of three related tribes of the basidiomycete family Tricholomataceae. The genomic data will be used to improve recognition of generic groups of these fungi and to determine the phylogenetic affinities among the genera and tribes. The fungi will be surveyed for phylogenetically useful restriction site and sequence variation at loci coding for ribosomal RNA. In addition, Dr. Vilgalys has adapted laboratory procedures for filamentous fungi which allow rapid, direct sequencing of RNA from isolates from crude cellular lysates. Further genetic analyses will provide information about the rate and direction of evolution within ribosomal genes of fungi.