Cell - Surface Interactions in Biotechnology and Biomedicine

培养的，依赖锚定的哺乳动物细胞和它们所培养的表面之间的相互作用将被研究。原代人内皮细胞和3T3细胞将在经过系统改变和仔细控制的表面上培养。甲基丙烯酸烷基衍生物的共聚物将被用作培养表面，以确定表面自由能和化学成分如羧基、叔胺和季胺对细胞粘附、细胞形态和细胞代谢的影响。将在显微镜下测量细胞形态，并将包括最大粘附力。特定细胞粘附分子的作用也将被研究。细胞代谢将通过测量细胞生长速率、细胞外基质的合成、生长因子的释放和花生四烯酸代谢物的释放来研究。这些研究对生物技术和生物医学应用细胞培养的进步具有重要意义。从生物技术上讲，哺乳动物细胞培养用于生产关键和复杂的药物，而它们的性能与生物反应器的表面特性有关，人们知之甚少。生物医学上，组织替代物需要将活细胞附着在一些合成支撑结构上，例如，血管细胞附着在合成管上作为人造血管。不可避免地，由于表面相互作用，细胞功能发生了一些变化，这可能包括组织替代的功能。

The interactions between cultured, anchorage-dependent mammalian cells and the surfaces upon which they are cultured will be investigated. Primary human endothelial cells and 3T3 cells will be cultured on surfaces whose properties have been systematically altered and carefully controlled. Copolymers of derivitized alkyl methacrylates will be used as the culture surface to determine the effect of surface free energy and chemical moieties such as carboxyl, tertiaryamine, and quaternary amine groups on cell adhesion, cell morphology, and cell metabolism. Cell morphology will be measured microscopically and will include the maximum force of adhesion. The role of specificcell adhesion molecules will also be investigated. Cell metabolism will be investigated by measuring the cell growth rate, the synthesis of extra cellular matrix, the release of growth factors, and there lease of arachidonic acid metabolites. These investigations are important to advances in applied cell culture, both for biotechnological and biomedical use. Biotechnologically, mammalian cell culture is used to produce critical and complex pharmaceuticals, and their performance with respect to the surface characteristics of the bioreactor is poorly understood. Biomedically, tissue replacements are desired which involve live cells attached to some synthetic support structure, for example, blood vessel cells attached to a synthetic tube to serve as an artificial blood vessel. Invariably, some alteration in cell function is observed as a result of the surface interactions, and this can comprise the function of the tissue replacement.