Calcium Regulation of Membrane Biogenesis in Fusogenic Carrot Protoplasts

这是一个关于磷酸肌苷研究的建议，这是目前与动物和植物细胞中的第二信使模型有关的一个非常感兴趣的领域。首席研究员已经证明了磷脂酶和磷酸肌肽在膜融合中的重要性。本项目将重点研究膜溶磷脂酰肌醇4-三磷酸（LPIP）及其合成途径、分解代谢、功能意义和调控机制。生化、免疫化学和分子技术将被用于实现目标。由于该项目将有助于确定促进膜融合的参数，它可能对生物技术产生重要影响。

This is is a proposal on phosphoinositide research that is currently an area of great interest in relation to second messenger models both in animal and plant cells. The Principal Investigator has already demonstrated the importance of phospholipase and phosphoinositide in membrane fusion. The current project will focus on membrane lysophatidylinositol 4- triphosphate (LPIP), its synthetic pathways and catabolism, its functional significance and regulatory mechanism. Biochemical, immunochemical and molecular techniques will be used to achieve the objectives. Since the project will help identify parameters that promote membrane fusion, it could have important ramifications in biotechnology.