Molecular Analysis of Arabidopsis Genes Involved in Responseto Abscisic Acid

脱落酸(ABA)调节植物生长发育的许多方面，但识别和细胞反应之间的连锁反应仍然不清楚。芬克尔斯坦博士的总体目标是识别参与ABA活动的蛋白质，并通过生理、生化和分子遗传学分析研究它们的功能。她正在使用对ABA敏感性改变的拟南芥突变体来识别与ABA反应有关的基因。芬克尔斯坦博士还分离出了与玉米ABA不敏感基因VP1同源的拟南芥基因组克隆。她建议通过以下方法来分析它们的功能：1)确定它们是否对应于任何ABI基因座；2)表征它们在野生型和ABI植物中表达的发育特异性；3)DNA序列分析和4)免疫定位。如果两个序列都不与任何ABI基因座共聚，她将尝试使用反义基因创造突变表型。植物生长调节剂脱落酸影响植物生长发育的许多方面，包括胚胎发育、种子休眠、水分关系、细胞伸长、对各种环境胁迫的耐受性和衰老。这项研究将确定一些与脱落酸作用有关的基因。

Abscisic acid (ABA) regulates many aspects of plant growth and development, but the chain of events between recognition and cellular response remains obscure. Dr. Finkelstein's overall objective is to identify proteins involved in ABA action and study their function by physiological, biochemical and molecular genetic analysis. She is using mutants of Arabidopsis with altered sensitivity to ABA to identify genes involved in ABA response. Dr. Finkelstein has also isolated Arabidopsis genomic clones with homology to the maize ABA-insensitive locus, vp1. She proposes to analyze their function by: 1) determining whether they correspond to any of the ABI loci; 2) characterizing the developmental specificity of their expression in both wild type and abi plants; 3) DNA sequence analysis and 4) immunolocalization. If neither sequence cosegregates with any abi locus, she will attempt to create a mutant phenotype using antisense genes. %%% THe plant growth regulator abscisic acid affects many aspects of a plants growth and development, including embryo development, seed dormancy, water relations, cell elongation, tolerance to a variety of environmental stresses and senescence. This study will identify some of the genes involved in abscisic acid action.