Acquisition of Preparative HPLC System

这是一个建议，以获得制备高效液相色谱系统，主要用于纯化合成肽。本提案中详细的科学部分描述了肽合成在每个研究者计划中的作用。通过制备高效液相色谱法纯化大量合成肽的能力对于继续进行(1)肽激素加工机制和激素前前体蛋白构象的研究(2)乙型肝炎病毒抗原结构的研究(3)和(4)肽中Asn和Gln残基的非酶脱酰胺机制的研究至关重要(5)蛇毒止血作用及合成抗凝肽的研究(6)合成生长激素片段促有丝分裂活性的研究(7)培养胶质细胞促有丝分裂因子的研究(8)体外和体内碱性磷酸酶修饰的研究。

This is a proposal to acquire a Preparative HPLC System to be used primarily for the purification of synthetic peptides. The science sections detailed in this proposal describe the role of peptide synthesis in each investigator's program. The ability to purify large (mg) quantities of the synthesized peptides by preparative HPLC is essential for continued (1) Studies on processing mechanisms of peptide-hormones and conformation of pro-hormone precursor proteins (2) Studies on the structure of hepatitis B viral antigens (3) and (4) Studies on the mechanism os nonenzymatic de-amidation of Asn and Gln residues in peptides and proteins (5) Studies on the effects of snake venoms on hemostasis and the development of synthetic anticoagulant peptides (6) Studies on the mitogenic activity of synthetic growth hormone fragments (7) Studies on mitogenic factors for cultured glial cells (8) Studies on the modification of alkaline phosphatase in vitro and in vivo.