Instrumentation for Research in Animal-Cell Culture and Metabolic Engineering

这项建议所要求的仪器将加强动物细胞培养工程和厌氧细菌代谢工程中的PI研究，使PI能够更密切地监测细胞新陈代谢和产品表达的变化。所要求的仪器最初将用于三个主要研究项目。第一个项目是研究不同环境压力对杂交瘤细胞产生单抗的影响。它还将研究应激蛋白启动子在动物细胞生产重组糖蛋白方面的效用。第二部分考察了生物反应器水动力环境对微载体和自由悬浮反应器中动物细胞的活性、代谢、大分子合成和产物表达的影响。第三个项目将研究改变丁酸厌氧细菌(梭状芽胞杆菌)初级代谢(代谢工程)的能力和方法，以构建更高效的全细胞生物催化剂，用于大规模厌氧发酵。

The instrumentation requested in this proposal will enhance the PIs research in animal-cell culture engineering and metabolic engineering of anaerobic bacteria by enabling the PIs to more closely monitor changes in cell metabolism and product expression. The requested instruments will be initially used for three major research projects. The first project investigates the effect of various environmental stresses on monoclonal antibody production by hybridoma cells. It will also examine the utility of stress-protein promoters for production of recombinant glycoproteins by animal cells. The second examines effects of the bioreactor hydrodynamic environment on the viability, metabolism, macromolecular synthesis, and product expression of animal cells in microcarrier and free-suspension reactors. The third project will investigate the ability and means of altering the primary metabolism (metabolic engineering) of butyric-acid anaerobic bacteria (clostridia) in order to construct more efficient whole-cell biocatalysts for large scale anaerobic fermentations.