Preparative High Performance Capillary Electrophoresis

高效毛细管电泳法(HPCE)在蛋白质和核酸的分析中具有传统凝胶电泳法无法比拟的优势。它比凝胶快得多，可以完全自动化，并通过样品注入、检测和收集进行仪器测量。此外，高效毛细管电泳法可以分离溶质，其分辨率比高效液相色谱高一到两个数量级，所用样本量比高效液相色谱仪小四个数量级。然而，单毛细管高效毛细管电泳法只能处理小于1ug的溶质载量。它不能用于预备规模，以提供进一步分析、临床研究或治疗应用的材料。大多数熟悉这项技术的科学家认为，如果HPCE能够扩大到准备水平，它将成为一种不可或缺的实验室工具。不幸的是，放大到制备型HPCE并非易事：制备型HPCE柱必须有效地散热，同时保持ug中常规观察到的极高的板效率。HPCE。为了达到1mgHPCE的目标，提出了单毛细管和多毛细管HPCE的理论分析和实验分析相结合的研究。本研究的目的是(1)阐明单个毛细管内的传热、分离和分散的机理，并将这些知识应用于制备规模的HPCE柱的设计。

High performance capillary electrophoresis (HPCE) offers important advantages over conventional gel electrophoresis in the characterization of proteins and nucleic acids. It is much faster than the gels and can be fully automated and instrumented with sample injection, detection and collection. In addition, HPCE can separate solutes with a resolution which is one to two orders of magnitude greater than high performance liquid chromatography (HPLC) using sample sizes four orders of magnitude smaller than HPLC. However, single-capillary HPCE can only handle solutes loadings of less than 1 ug. It cannot be used at preparatory scales to provide material for further analysis, clinical research or therapeutic application. Most scientists familiar with this technology believe that, if HPCE could be scaled to preparative levels, it would become an indispensable laboratory tool. Unfortunately, scale-up to preparative HPCE is not simple: a preparative HPCE column must dissipate Joule heat effectively while maintaining the extraordinarily high plate efficiency routinely observed in ug. HPCE. To reach the goal of 1 mg HPCE, a study is proposed which combines both theoretical and experimental analyses of single and multiple capillary HPCE. The objectives of this research are to (1) elucidate the mechanisms of heat transfer, separation and dispersion in single capillaries and to apply this knowledge in the (2) design of a preparative-scale HPCE column.