Reduced one Carbon Biosynthetic Pathways of the Methanogen Methanosphaera stadtmanae

该项目将研究一种具有不同寻常生理特性的产甲烷菌——它既不能用二氧化碳也不能用醋酸盐生产甲烷，但只要有氢气存在，就可以用甲醇生产甲烷。令人惊讶的是，细胞成分的大部分碳来自醋酸盐或二氧化碳，很少来自甲醇；生物合成的后一个方面是研究的重点，研究将使用13-C标记的醋酸盐或二氧化碳，然后进行高分辨率核磁共振研究，利用完整的细胞和细胞提取物阐明关键的碳入口途径。关键的酶反应和辅酶需求的明显不寻常的还原一碳生物合成途径(s)的中心反应也将被研究。研究一种生理上不寻常的产甲烷古细菌，目的是了解醋酸盐和二氧化碳碳被构建到细胞成分中的方式，这是该项目的重点，涉及微生物学家和化学家之间的合作。//

This project will study a methanogen with unusual physiological attributes--the inability to produce methane from either carbon dioxide or acetate, but to do so from methanol provided that hydrogen is present. Surprising is the fact that a great deal of carbon of cell components is derived from acetate or carbon dioxide and little from methanol; it is this latter aspect of biosynthesis that is the focus of investigations that will use 13-C labelled acetate or carbon dioxide, followed by high-resolution NMR studies, to elucidate key carbon-entrance pathways using intact cells and cell extracts. Key enzymatic reactions and coenzyme requirements of central reactions of the apparently unusual reduced one-carbon biosynthetic pathway(s) will also be studied. The study of a physiologically-unusual methanogenic archaebacterium, with the goal of understanding the means by which acetate and carbon dioxide carbon are built into cellular components is the focus of this project which involves collaboration between microbiologists and chemists. //