Acquisition of an X-ray Area Detector for Macromolecular Crystallography

要求美国国家科学基金会提供配套资金，以帮助洛克菲勒大学两个独立的结晶学小组共享一台成像板X射线区域探测器。由约翰·库里扬和斯蒂芬·K·伯利领导的这两个小组正在探索大分子的三维结构与其生物功能之间的内在联系。与洛克菲勒和其他地方的研究小组合作，正在进行的项目包括：(1)黄蛋白二硫化物还原酶(J.K.)，(2)细菌氧化应激传感器和氧化应激诱导酶的转录调节(J.K.)，(3)果蝇发育基因产物(J.K.)，(4)真核转录因子IID或TATA因子(S.K.B.)；(5)真核转录上游刺激因子(S.K.B.)。已经购买了一台旋转阳极X射线发生器和一台高速4圈单计数器衍射仪以及相关的计算设备。购置拟议的区域探测器将产生最先进的X射线数据收集设施。

NSF matching funds are requested to help purchase an Imaging Plate X-ray Area Detector for shared use by two independent crystallography groups at the Rockefeller University. These two groups, headed by John Kuriyan and Stephen K. Burley, are exploring the intrinsic link between the three-dimensional structures of macromolecules and their biological function. In collaboration with groups at Rockefeller and elsewhere, the projects underway include structure-function studies on: (1) flavo-protein disulfide reductases (J.K.), (2) bacterial oxidative stress sensors and transcriptional regulation of oxidative stress-induced enzymes (J.K.), (3) developmental gene products in Drosophila (J.K.), (4) eukaryotic transcription factor IID or TATA factor (S.K.B.), (5) eukaryotic transcriptional upstream stimulator factor (S.K.B.). A rotating- anode X-ray generator and a high speed 4-circle single counter diffractometer have already been purchased, along with associated computing equipment. Acquisition of the proposed area detector will result in a state-of-the-art X-ray data collection facility.